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Position: Home > Articles > Ecological distribution of Listeria spp.in healthy sheep and surrounding environments in Xinjiang Animal Husbandry & Veterinary Medicine 2012,44 (1) 28-32

新疆绵羊及其环境中李氏杆菌的生态分布多样性调查

作  者:
赵新斐;尚文婧;王静梅;剡根强
单  位:
石河子大学动物科技学院
关键词:
李氏杆菌;生态分布;绵羊;环境
摘  要:
对新疆健康绵羊及其相关环境如青贮饲料、饮水、土壤、鸟粪中李氏杆菌(Listeria)种群采用改良国标法(4789.30-2010)进行生态分布多样性调查,并对分离的单核细胞增多性李氏杆菌(L.monocytogenes)采用PCR方法进行溶血素基因(hly)和血清型检测。结果:514份样本中,共检出阳性样本26份,平均阳性率为5.1%,其中羊体分离率为6.2%(22/353),饮水分离率为33%(2/6),鸟粪分离率为3.0%(2/67),其余样本均为阴性;26份阳性样品中,检出单核细胞增多性李氏杆菌5株,塞氏李氏杆菌(L.seeligeries)21株,且5株单核细胞增多性李氏杆菌均检出溶血素基因,血清型为1/2a。
译  名:
Ecological distribution of Listeria spp.in healthy sheep and surrounding environments in Xinjiang
作  者:
ZHAO Xin-fei,SHANG Wen-jing,WANG Jing-mei,YAN Gen-qiang(College of Animal Science and Technology,Shihezi University,Shihezi 832000,China)
关键词:
Listeria;ecological distribution;sheep;surrounding environments
摘  要:
A survey on ecological distribution of Listeria spp.in healthy sheep and surrounding environments such as silage,water,soil and bird feces,was performed using the modified GB method(4789.30-2010) in Xinjiang.For the L.monocytogenes isolates,the hemolysin gene(hly) and serotypes were investigated by PCR method.The result showed that among 514 samples,there were 26 positive specimens,and the average positive rate was 5.1%.The positive rates in sheep,water and bird feces were 6.2%(22/353),33%(2/6) and 3%(2/67),respectively.The remaining samples were negative.Among 26 positive samples,5 were L.monocytogenes and 21 were L.seeligeries.All the five strains of L.monocytogenes were detected hly gene and determined to be the serotype 1/2a.

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