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当前位置: 首页 > 文章 > 伊犁地区绵羊李氏杆菌药敏特性及平板凝集快速诊断方法的建立 安徽农业科学 2014 (2) 480-482
Position: Home > Articles > Drug Sensitivity Characteristics of Listeria monocytogenes and Establishment of Plate Agglutination Fast Diagnostic Technique in Yili Region Journal of Anhui Agricultural Sciences 2014 (2) 480-482

伊犁地区绵羊李氏杆菌药敏特性及平板凝集快速诊断方法的建立

作  者：
皮志媛;王一明;张海兰;雷程红
单  位：
新疆农业大学;伊犁职业技术学院
关键词：
李氏杆菌;药敏特性;快速诊断方法
摘  要：
[目的]建立一种快速检测李氏杆菌病的方法。[方法]通过对实验室保存的5株李氏杆菌分离株进行药敏试验,用甲醛灭活苗免疫试验动物,制备特异性抗体,将特异性抗体与抗原进行平板凝集试验。[结果]分离菌株对氨苄西林、庆大霉素、链霉素高度敏感,对头孢唑啉、左氧氟沙星和多粘菌素中度敏感,对头孢他啶、头孢呋辛钠耐药性较强。通过用甲醛灭活苗免疫试验动物来制备特异性抗体,将特异性抗体与抗原做平板凝集试验,建立一种快速准确检测李氏杆菌病的方法。[结论]成功建立了一种快速检测李氏杆菌病的方法。
译  名：
Drug Sensitivity Characteristics of Listeria monocytogenes and Establishment of Plate Agglutination Fast Diagnostic Technique in Yili Region
作  者：
PI Zhi-yuan;Xinjiang Agricultural University;
关键词：
Listeria monocytogenes;;Drug sensitivity characteristics;;Rapid diagnostic method
摘  要：
[Objective] The research aimed to establish a rapid determination method of listeriosis. [Method]Five strains of Listeria monocytogenes preserved in the laboratory were use to make drug sensitivity test. The test animals were immunized by using formaldehyde-inactivated vaccine to make specific antibody. The specific antibody and antigen were used to make plate agglutination test. [Result]The isolated strains were highly-sensitive to ampicillin,gentamicin and streptomycin,moderately-sensitive to cefazolin,levofloxacin and polymyxin. And the isolated strains were highly-toleratn to ceftazidime and cefuroxime sodium. The test animals were immunized by formaldehyde-inactivated vaccine to make specific antibody. The specific antibody and antigen was used to make plate agglutination test. Thus a rapid and accurate detection method of listeriosis was established. [Conclusion] A rapid detection method of listeriosis was successfully established.

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