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当前位置: 首页 > 文章 > 大孔树脂固定化磷脂酶A_1及其用于菜籽油脱胶工艺的优化 食品科学 2018 (4) 241-247
Position: Home > Articles > Optimization of Process Parameters for Immobilization of Phospholipase A_1 onto Macroporous Resin and Its Application for Degumming Rapeseed Oil FOOD SCIENCE 2018 (4) 241-247

大孔树脂固定化磷脂酶A_1及其用于菜籽油脱胶工艺的优化

作  者：
水龙龙;操丽丽;庞敏;潘丽军;鲍赛;李进红;姜绍通
单  位：
合肥工业大学食品科学与工程学院安徽省农产品精深加工重点实验室
关键词：
磷脂酶A1;固定化;大孔树脂;脱胶
摘  要：
选择8种大孔树脂对磷脂酶A_1进行固定化,结果表明,离子交换树脂D001的固定化效果最好,其优化的最佳条件为缓冲液p H 5.0、酶添加量1.5 m L/g、固定化时间4 h,在该条件下获得的固定化酶活力为665.8 U/g。将固定化酶用于菜籽油脱胶实验,经响应面优化确定最优脱胶条件为固定化酶添加量1.8 g/kg、反应时间3.6 h、反应温度51℃、反应p H 5.5,在此条件下得到的脱胶油中磷含量为5.82 mg/kg。将固定化酶重复脱胶5次后,仍保留初始酶活力的47.9%,脱胶油中磷含量为9.78 mg/kg。
译  名：
Optimization of Process Parameters for Immobilization of Phospholipase A_1 onto Macroporous Resin and Its Application for Degumming Rapeseed Oil
作  者：
SHUI Longlong;CAO Lili;PANG Min;PAN Lijun;BAO Sai;LI Jinhong;JIANG Shaotong;Key Laboratory for Agriculture Products Processing of Anhui Province,School of Food Science and Engineering, Hefei University of Technology;
关键词：
phospholipase A1;;immobilization;;macroporous resin;;degumming
摘  要：
Eight kinds of macroporous resins were comparatively used to immobilize phospholipase A_1, and the best carrier was found to be D001. The optimum conditions for immobilization were as follows: p H 5.0, phospholipase dosage 1.5 m L/g and 4 h reaction, yielding an activity of immobilized enzyme as high as 665.8 U/g. The degumming of rapeseed oil with the immobilized enzyme was optimized using response surface methodology. The optimum conditions were determined as follows: immobilized enzyme dosage 1.8 g/kg, p H 5.5, reaction time 3.6 h, and reaction temperature 51 ℃. The phosphorus content of degummed oil prepared under these condition was 5.82 mg/kg. After fifth repeated use, the immobilized enzyme still retained 47.9% of its initial activity and the phosphorus content of degummed oil was 9.78 mg/kg.

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