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Position: Home > Articles > Genetic Diversity Among 18 Xiang-hui-jun Strains Analyzed Using RAPD,SRAP and ISSR Markers Acta Edulis Fungi 2014 (2) 25-31

具有拮抗关系的18个香灰菌株遗传差异性分析

作  者:
邓优锦;王庆福;陈介斌;苏婉真;谢宝贵
单  位:
福建农林大学菌物研究中心
关键词:
香灰菌;RAPD;SRAP;ISSR
摘  要:
香灰菌(xiang-hui-jun)是银耳(Tremella fuciformis)的伴生菌,对银耳的生长、发育具有至关重要的作用。采用RAPD、SRAP和ISSR三种分子标记对具有拮抗关系的18个香灰菌菌株进行遗传差异性分析。结果表明:8对RAPD引物共扩增出59个位点片段,多态性条带总共为49条,占总片段条数的比率为83%;7对SRAP引物共扩增出49个位点片段,多态性条带总共为47条,占总片段条数的比率为96%;6对ISSR引物共扩增出60个位点片段,多态性条带总共为50条,占总片段条数的比率为83%。聚类分析表明,当以相异系数0.48为阈值时,可将这些香灰菌株分成7个类群:Hp.sp0006、TR柏溪、瓦坑野生、TR枫树和TR8801枫树分别自成一个类群,Hp.sp0052和69为一个类群,其余11个(Hp.sp0004、Hp.sp0010、T0050、T0048、T0036、TR23、TR5、TR95、71、TR03和TR86)菌株归为一个类群。
译  名:
Genetic Diversity Among 18 Xiang-hui-jun Strains Analyzed Using RAPD,SRAP and ISSR Markers
作  者:
DENG Youjin;WANG Qingfu;CHEN Jiebin;SHU Wanzhen;XIE Baogui;Mycological Research Center,Fujian Agriculture and Forestry University;
关键词:
Xiang-hui-jun;;RAPD;;SRAP;;ISSR
摘  要:
Genetic diversity among 18 antagonistic strains of Xiang-hui-jun,the cohabitant fungus of Tremella fuciformis that plays a vital role in the growth and development of the mushroom,was analyzed using randomly amplified polymorphic DNA(RAPD),sequence-related amplified polymorphism(SRAP)and intersimple sequence repeat(ISSR)markers.Eight RAPD primers generated 59 DNA bands from the 18 strains of which 49(83%)were polymorphic,seven SRAP primers generated 49 DNA bands of which 47(96%)were polymorphic,while six ISSR primers generated 60 DNA bands of which 50(83%)were polymorphic.A dendrogram based on RAPD、ISSR and RAPD analyses was constructed using the unweighted pair-group method with arithmetic means,and the 18 strains were delineated into seven groups at a dissimilarity coefficient level of 0.48 as follows:strains Hp.sp0006,TR Boxi,Wakeng wild,TR Fengshu and TR8801 Fengshu each constituted individual groups(Groups 1-5),Group 6comprised strains Hp.sp0052 and 69,and the remaining 11strains(Hp.sp0004,Hp.sp0010,T0050,T0048,T0036,TR23,TR5,TR95,71,TR03 and TR86)clustered together to form Group 7.

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