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当前位置: 首页 > 文章 > β-甘露聚糖酶产生菌HDYM-04的分离鉴定及产酶条件优化 食品科学 2009,30 (21) 262-266
Position: Home > Articles > Isolation and Identification of Beta-Mannanase Producing Strain HDYM-04 and Optimization of Fermentation Conditions FOOD SCIENCE 2009,30 (21) 262-266

β-甘露聚糖酶产生菌HDYM-04的分离鉴定及产酶条件优化

作  者：
葛菁萍;赵丹;宋刚;凌宏志;平文祥
单  位：
微生物黑龙江省高校重点实验室黑龙江大学生命科学学院
关键词：
β-甘露聚糖酶;16SrDNA;优化;正交试验
摘  要：
从实验室温水沤麻液中分离并筛选到了一株产β-甘露聚糖酶的细菌HDYM-04,经生理生化实验及16SrDNA序列分析鉴定为地衣芽孢杆菌(Bacillus licheniformis)。研究HDYM-04菌株72h连续发酵过程中产酶及生长的动态变化,通过单因素及序贯正交试验优化得到其摇瓶发酵产酶的最佳培养基组分及条件:碳源60g/L魔芋粉、氮源30g/L蛋白胨、MgSO4·7H2O0.2g/L、K2HPO45g/L、初始pH8.0、装液量60ml/250ml三角瓶、接种量2%、37℃振荡培养48h。在研究条件下发酵液酶活力为4890U/ml,比优化前提高了3.2倍。
译  名：
Isolation and Identification of Beta-Mannanase Producing Strain HDYM-04 and Optimization of Fermentation Conditions
作  者：
GE Jing-ping,ZHAO Dan,SONG Gang,LING Hong-zhi,PING Wen-xiang (Key Laboratory of Microbiology of Heilongjiang Province, Life Science College, Heilongjiang University, Harbin 150080, China)
关键词：
β-mannanase;16S rDNA;optimization;orthogonal array design
摘  要：
A β-mannanase producing bacterial strain HDYM-04 was isolated from laboratory-prepared flax retting liquor. HDYM-04 was identified as Bacillus licheniformis according to physiological and biochemical tests and phylogenetic analysis based on16S rDNA sequence. Single factor and orthogonal array design experiments were performed to obtain the optimum fermentation conditions for β-mannanase production: culture with an inoculation size of 2% for 48 h at 37 ℃ in a 250 ml shaking flask filled with 100 ml liquid medium consisting of 60 g/L konjac powder utilized as carbon source, 30 g/L peptone utilized as nitrogen source, 0.2 g/L MgSO4·7H2O, 5 g/L K2HPO4 at initial pH 8.0. Under these optimum conditions, a β-mannanase activity of 4890 U/ml fermentation broth was achieved, which was 3.2 times higher than before the optimization.

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