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当前位置: 首页 > 文章 > 黑果小檗中总黄酮的超声提取及其含量测定 安徽农业科学 2012,40 (14) 212-213
Position: Home > Articles > Extraction of Total Flavonoids in Breberis heteropoda Shrenk by Ultrasonic and Determination of Its Content Journal of Anhui Agricultural Sciences 2012,40 (14) 212-213

黑果小檗中总黄酮的超声提取及其含量测定

作  者：
刘河疆;钱宗耀;王建梅;周晓龙
单  位：
新疆农业科学院中心实验室
关键词：
黑果小檗;总黄酮;芦丁;分光光度法;超声提取
摘  要：
[目的]建立一种测定黑果小檗中总黄酮含量的方法。[方法]用超声波提取黑果小檗果实中的总黄酮,然后以芦丁为标准品,采用分光光度法测定总黄酮含量。[结果]芦丁浓度与吸光度的线性关系方程为A=0.010 74 C+0.009 214(r=0.999 2),表明芦丁在7.5～60.0μg/ml范围内与吸光度值线性关系良好,平均回收率为93.6%;测得黑果小檗果肉、籽粒中总黄酮的含量分别为0.78%和1.89%。[结论]该方法快速、简便、可靠,可用于黑果小檗中总黄酮含量的测定。
译  名：
Extraction of Total Flavonoids in Breberis heteropoda Shrenk by Ultrasonic and Determination of Its Content
作  者：
LIU He-jiang et al(Center Laboratory of Xinjiang Academy of Agricultural Science,Urmqi,Xinjiang 830091)
关键词：
Breberis heteropoda Shrenk;Total flavonoids;Rutin;Spectrophotometry;Ultrasonic extraction
摘  要：
[Objective] The aim was to establish a method to determine total flavonoids content in Breberis heteropoda Shrenk.[Method] Ultrasonic technology was used to extract the total flavonoid of the fruits of B.heteropoda Shrenk,and then with rutin as the standard substance,total flavonoids were determined by spectrophotometry.[Result] The results showed that the linear relationship equation of rutin concentration and the absorbance was A=0.010 74 C+0.009 214(r=0.999 2),which indicated that there was a good linear relationship between the absorbance and the concentration of rutin within the range of 7.5~60.0 μg/ml.The average recovery rate was 93.6%,and the contents of total flavonoids in pulp and seed were 0.78% and 1.89% respectively.[Conclusion] The method was found to be fast,simple,reliable and good for the determination of total flavonoids in B.heteropoda Shrenk.

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