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Position: Home > Articles > Determination of DHA and Linoleic Acid Contents in Infant Formula Milk Powder by GC-MS FOOD SCIENCE 2011,32 (14) 261-263

GC-MS法测定婴幼儿配方奶粉中DHA和亚油酸含量

作  者:
彭飞进;杨卫花
单  位:
大理州质量技术监督综合技术检测中心
关键词:
二十二碳六烯酸(DHA);亚油酸;婴幼儿配方奶粉;气相色谱-质谱联用(GC-MS)
摘  要:
建立一种气相色谱-质谱联用(GC-MS)法测定婴幼儿配方奶粉中二十二碳六烯酸(DHA)和亚油酸含量的方法。乙酰氯-甲醇将样品中DHA和亚油酸甲酯化,用甲苯提取,经GC-MS法测定及确证,外标法定量。结果表明,添加回收率范围为96%~110%,精密度高。该方法简便,测定结果准确可靠,完全适合婴幼儿配方奶粉中DHA和亚油酸的分析检测。
译  名:
Determination of DHA and Linoleic Acid Contents in Infant Formula Milk Powder by GC-MS
作  者:
PENG Fei-jin,YANG Wei-hua(Comprehensive Technical Inspection Center,Dali Quality and Technical Supervision,Dali 671000,China)
关键词:
docosahexaenoic acid(DHA);linoleic acid;infant formula milk powder;gas chromatography-mass spec-trometry(GC-MS)
摘  要:
A method to determine docosahexaenoic acid(DHA) and linoleic acid contents in infant formula milk powder by gas chromatography-mass spectrometry(GC-MS) was developed.Samples were methylesterized with acetyl chloride-MeOH,followed by extraction with toluene before GC-MS analysis.The external standard method was used for quantification.The average spike recoveries for DHA and linoleic acid in a commercial sample were 96%-110%.The developed method proved simple,precise,accurate and reliable,thereby being suitable for the determination of DHA and linoleic acid contents in infant formula milk powder.

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