当前位置: 首页 > 文章 > 大叶藜叶中总黄酮含量的测定 安徽农业科学 2015 (34) 164-165
Position: Home > Articles > Determination of Total Flavone in the Leaf of Chenopodium hybridum L. Journal of Anhui Agricultural Sciences 2015 (34) 164-165

大叶藜叶中总黄酮含量的测定

作  者:
武芸;马世荣;王春林;胡浩斌;张小伟
单  位:
陇东学院化学化工学院;陇东学院生命科学与技术学院
关键词:
紫外-可见分光光度法;大叶藜;总黄酮;含量测定
摘  要:
[目的]建立大叶藜叶中总黄酮含量的测定方法。[方法]采用分光光度法,以芦丁为对照品,10%KOH为显色剂,在403 nm处对样品中的总黄酮含量进行测定。[结果]总黄酮在10.7~64.2μg/ml范围内呈良好的线性关系,R~2=0.997;平均加样回收率为98.45%,RSD为0.56%(n=6),大叶藜叶中的总黄酮含量为2.45 mg/g。[结论]该方法稳定、简便、快速,适用于大叶藜叶中总黄酮含量的测定。
译  名:
Determination of Total Flavone in the Leaf of Chenopodium hybridum L.
作  者:
WU Yun;MA Shi-rong;WANG Chun-lin;College of Chemistry and Chemical Engineering,Longdong University;College of Life Science and Technology,Longdong University;
关键词:
UV-visble spectrophotometry;;Chenopodium hybridum;;Total flavonoids;;Content determination
摘  要:
[Objective] To establish a method for the total flavonoid content determination of the leaf of Chenopodium hybridum L.[Method]The content of total flavones from the leaf of Chenopodium hybridum L. was determined by UV-visble spectrophotometry with liquiritin as reference substance,10% KOH as chromogenic agent at the detection wavelength of 403 nm. [Result]The total flavonoid showed a good linear relationship at the range of 10. 7-64. 2 μg / ml,R~2= 0. 997. The average recovery rate was 98. 45%,RSD = 0. 56%. Total flavone content in the leaf of Chenopodium hybridum was 2. 45 mg / g. [Conclusion]The method was ralatively simple,convenient and accurate,and could be used for the quality control for Chenopodium hybridum L.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊