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Position: Home > Articles > HPLC Determination of Melamine in Milk Product FOOD SCIENCE 2009,30 (24) 385-388

高效液相色谱法测定奶制品中的三聚氰胺

作  者:
刘立萍
单  位:
怀化市质量技术监督局产商品质量监督检验所
关键词:
三聚氰胺;高效液相色谱;奶制品
摘  要:
建立高效液相色谱法检测乳制品中三聚氰胺的新方法。用乙腈-三氯乙酸溶液提取样品中的三聚氰胺,混合型阳离子交换柱进行净化。色谱柱ThermoC8(150mm×4.6mm,5μm),流动相为乙腈-缓冲液(庚烷磺酸钠-柠檬酸),检测波长240nm,检出限为0.15μg/ml。加标回收率为86.39%~102.60%。结果准确可靠,仪器要求简单,方法易于推广,通过了国家认证认可。
译  名:
HPLC Determination of Melamine in Milk Product
作  者:
LIU Li-ping (Product Commodity Quality Supervision Testing Institute, The Quality and Technology Supervision Bureau of Huaihua, Huaihua 418000, China)
关键词:
melamine;HPLC;milk product
摘  要:
Melamine in milk products was extracted by acetonitrile-trichloroacetic acid solution, and purified by mixed positive ion exchange column, followed by HPLC determination. Melamine was separated by a DBS-C8 column (4.6×150 mm, 5 μm, Thermo) using acetonitrile-cushion solution (1-pentan esulfonic acid sodium salt-citric acid monohydrate) as mobile phase with the flow rate at 1.0 ml/min, and detected at 240 nm. The limit of detection was 0.15μg/ml with the average recovery between 86.39% and 102.60%.This method has been notarized by national authentication committee.

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