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当前位置: 首页 > 文章 > 基于信号增敏型试纸条三聚氰胺超灵敏检测方法 食品科学 2014,35 (8) 289-294
Position: Home > Articles > A Novel Ultrasensitive Method for Detection of Melamine Based on Signal Amplified Lateral Flow Chromatographic Strips FOOD SCIENCE 2014,35 (8) 289-294

基于信号增敏型试纸条三聚氰胺超灵敏检测方法

作  者：
钟友好;赵弟萍;薛峰;朱梦雅;方威;陈伟
单  位：
合肥工业大学生物与食品工程学院
关键词：
胶体金;三聚氰胺;增敏;免疫层析
摘  要：
通过胶体金免疫层析技术建立一种能便捷、快速、特异检测食品中三聚氰胺的超灵敏快速检测技术。在传统的基于竞争法层析试纸条的基础上增加一个增强垫组装成增敏型试纸条,在识别垫和增强垫上分别使用25 nm和15 nm胶体金,使其在传统型试纸条的基础上对三聚氰胺的检测灵敏度大大提高。结果表明:增敏后的试纸条可在10 min内实现食品中三聚氰胺的快速检测;实验条件下检测灵敏度为0.5μg/L,是传统检测灵敏度10μg/L的20倍;实际样品中检测范围为0.1~5μg/L,检测灵敏度为1μg/L,相比于传统检测灵敏度提高了5倍。
译  名：
A Novel Ultrasensitive Method for Detection of Melamine Based on Signal Amplified Lateral Flow Chromatographic Strips
作  者：
ZHONG You-hao;ZHAO Di-ping;XUE Feng;ZHU Meng-ya;FANG Wei;CHEN Wei;School of Biotechnology and Food Engineering, Hefei University of Technology;
关键词：
colloidal gold nanoparticle;;melamine detection;;lateral flow strip;;signal amplification
摘  要：
T his paper reports on a convenient and rapid method for the detection of melamine in food samples by lateral fl ow strip technology. The method is based on competitive immunoreaction. The traditional test strip was made much more sensitive by adding an enhancement pad. For the melamine lateral fl ow strip, 25 nm and 15 nm colloidal gold nanoparticles were adopted in the recognition and enhancement pads, respectively. The results showed that detection of melamine in food samples could be achieved in less than 10 min. The limit of detection(LOD) of the enhanced lateral fl ow strip was 0.5 μg/L under our experimental conditions, which showed a 20-fold increase over 10 μg/L for the traditional lateral fl ow strip. This new method was able to detect 0.1–5 μg/L melamine in real samples and the sensitivity was 1 μg/L, indicating a 5-fold increase over that of the traditional one.

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