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当前位置: 首页 > 文章 > 粒毛盘菌DP5胞外多酚的提取及抗氧化活性研究 安徽农业科学 2015 (17) 12-14+114
Position: Home > Articles > Extractionand Antioxidant Activity of Extracellular Polyphenols from Lachnum DP5 Journal of Anhui Agricultural Sciences 2015 (17) 12-14+114

粒毛盘菌DP5胞外多酚的提取及抗氧化活性研究

作  者：
曹焕英;孙丹宇;杨柳;钱潇;牛兆众
单  位：
合肥工业大学生物与食品工程学院
关键词：
粒毛盘菌;胞外多酚;提取;抗氧化活性
摘  要：
[目的]使用树脂对粒毛盘菌DP5胞外多酚进行提取,并且对其体外抗氧化活性进行评价。[方法]以多酚得率为指标,优化粒毛盘菌DP5胞外多酚的提取工艺。[结果]以大孔树脂X-5为填充料,进样浓度为0.64 mg/ml,进样流速为1 ml/min,洗脱溶剂为浓度80%的乙醇,洗脱流速为1 ml/min,粒毛盘菌胞外多酚含量为815.7 mg GAE/g。体外抗氧化试验表明,粒毛盘菌DP5胞外多酚具有还原力和清除DPPH自由基、羟自由基(·OH)、亚硝酸盐(NO2-)的效果,且随着多酚浓度的升高其活性增强。[结论]该研究结果为粒毛盘菌多酚的开发利用提供理论依据。
译  名：
Extractionand Antioxidant Activity of Extracellular Polyphenols from Lachnum DP5
作  者：
CAO Huan-ying;SUN Dan-yu;YANG Liu;School of Biotechnology and Food Engineering,Hefei University of Technology;
关键词：
Lachnum;;Extracellular polyphenol;;Extraction;;Antioxidant activities
摘  要：
[Objective] The study was to optimize the extraction process of the extracellular polyphenols from Lachnum DP5( LEP) using resin,and investigate its antioxidant activities in vitro. [Method] LEP were extracted and optimal conditions were investigated using single factor experiments. [Result] Macroporous resin X-5 was selected and the extraction conditions were as following: sample concentration of 0. 64 mg / ml,the injection flow rate of 1 ml / min,elution solvent of 80% ethanol,the elution flow rate of 1 ml / min. The contend of LEP reached815. 7 mg GAE / g under the optimal conditions. Antioxidant activity analysisin vitro for LEP extraction indicated that the LEP have the ability to scavengingfree radical of DPPH,hydroxyl,NO2-and restoring and the activity was related to its concentrations. [Conclusion]This study was aimed to provide theoretical basis for the development and utilization of LEP.

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