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当前位置: 首页 > 文章 > 辛格粒毛盘菌胞内多酚提取及其抗氧化性研究 食品科学 2008,29 (8) 249-252
Position: Home > Articles > Study on Intracellular Polyphenol Extraction from Lachnum singerianum and Its Antioxidant Activities FOOD SCIENCE 2008,29 (8) 249-252

辛格粒毛盘菌胞内多酚提取及其抗氧化性研究

作  者：
叶明;朱立
单  位：
合肥工业大学生物与食品工程学院
关键词：
粒毛盘菌;多酚;提取;抗氧化活性
摘  要：
在单因素试验的基础上,进行正交试验,以最大吸收波长下乙醇提取液吸光度为指标,探讨辛格粒毛盘菌胞内多酚(YM-82多酚)的提取工艺。所得最佳提取条件为:乙醇浓度80%、液料比50:1、时间9h。对YM-82多酚的抗氧化性进行研究,结果表明:浓度为21μg/ml的YM-82多酚,其还原能力强于25mg/ml的VC对照;浓度为24.7μg/ml的YM-82多酚对DPPH·清除率达到33.08%,为30mg/ml VC对照的1.18倍;浓度为22μg/ml的YM-82多酚对·OH清除率达到63.8%,远高于50mg/ml VC(对照),显示出YM-82多酚具有很强的抗氧化能力。
译  名：
Study on Intracellular Polyphenol Extraction from Lachnum singerianum and Its Antioxidant Activities
作  者：
YE Ming,ZHU Li(School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China)
关键词：
Lachnum singerianum;polyphenol;extraction;antioxidant activity
摘  要：
On the basis of single factor test,the extraction process of polyphenol from filamentous fungi Lachnum singerianum YM-82 was researched by orthogonal test through the absorbance determined at the typical peak. The results showed that the optimal conditions are as follows:concentration of ethanol 80%,ratio of liquid to solid 50:1 and time 4 h. The antioxidant and free radical scavenging activities of polyphenol from L. singerianum YM-82 were evaluated using hydroxyal radical,superoxide anion radical and 1,1-diphenyl-2-picrylhydrazyl(DPPH) in vitro model systems. The results showed that the polyphenol has strong antioxidant and free radical scavenging activities. The reducing power of the polyphenol of 21 μg/ml is stronger than that of 25 mg/ml VC. The clearance rate to DPPH· of the polyphenol is 33.08% at 24.7 μg/ml concentration,1.18 times of that of 30 mg/ml VC,and the clearance rate to hydroxyal radical of the polyphenol is 63.8% at 22 μg/ml concentration,significantly higher than that of 50 mg/ml VC system.

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