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当前位置: 首页 > 文章 > 禾本科粒毛盘菌多糖提取及其抗氧化活性研究 浙江大学学报(农业与生命科学版) 2009,35 (2) 153-157
Position: Home > Articles > Study on extraction technology and antioxidative activity of polysaccharides produced by Lachnum hyalopus. Journal of Zhejiang University (Agriculture and Life Sciences) 2009,35 (2) 153-157

禾本科粒毛盘菌多糖提取及其抗氧化活性研究

作  者：
叶明;李世艳;杨柳;朱立;林英任
单  位：
安徽农业大学;合肥工业大学
关键词：
粒毛盘菌;多糖;正交试验;抗氧化活性
摘  要：
对禾本科粒毛盘菌(Lachnumhyalopus)胞内多糖的微波提取工艺进行研究,并研究该多糖对超氧阴离子自由基()和1,1-二苯基苦基苯肼(DPPH)自由基的清除作用.结果表明,粒毛盘菌胞内多糖的最佳提取工艺条件为:微波功率150W,时间3min,液料比80,此条件下多糖得率可达12.05%.粒毛盘菌多糖对和DPPH自由基均有较好的清除作用,多糖浓度为100mg·L-1时,对的清除率达到86.67%;浓度为10mg·L-1时,对DPPH自由基的清除率达到10.79%.表明粒毛盘菌多糖具有明显的抗氧化活性.
译  名：
Study on extraction technology and antioxidative activity of polysaccharides produced by Lachnum hyalopus.
作  者：
YE Ming1,LI Shi-yan1,YANG Liu1,ZHU Li1,LIN Ying-ren2(1.College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China;2.College of Forestry and Garden,Anhui Agricultural University,Hefei 230036,China)
关键词：
Lachnum hyalopus;polysaccharide;orthogonal experiment;antioxidative activity
摘  要：
Extraction of polysaccharide from Lachnum hyalopus with microwave and its scavenging capacity of superoxide anion radical and 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical were studied.The results indicate that the optimum conditions for extraction of polysaccharide were as follows:microwave power was 150 W with ratio of liquid to material at 80 and 3 min of extraction time.Under above conditions,the polysaccharide yield was up to 12.05%.Meanwhile,the scavenging effects of the polysaccharide on  and DPPH free radicals were both significant,which the scavenging effects on the  radical reached 86.67% with 100 mg·L-1 polysaccharide,and the clearance rate of DPPH free radical reached 10.79% with 10 mg·L-1 polysaccharide.So it can be concluded that the polysaccharide displayed obvious antioxidant activities.

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