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Position: Home > Articles > Study on Isolation and Culture Characteristics of Paphiopedilum micranthum Mycorrhizal Fungi Northern Horticulture 2012 (7) 69-72

硬叶兜兰菌根真菌的分离及培养特性研究

作  者:
田凡;朱国胜;桂阳;白新祥
单  位:
贵州省农业科学院;贵州大学;贵州省农业生物技术重点实验室;贵州省现代中药材研究所
关键词:
硬叶兜兰;菌根真菌;菌丝团;分离;培养
摘  要:
采用单菌丝团法分离硬叶兜兰菌根真菌,纯化后,用打孔法培养菌株并观察形态特征并测定生长速度,初步鉴定了8株内生真菌(DJYYDL001、DJYYDL003、DJYYDL004、DJYYDL006、DJYYDL008、DJYYDL009、DJYYDL011、DJYYDL015)。结果表明:单菌丝团法分离出的菌丝团在恒温培养12h后镜检及吸取萌发菌丝团为最佳时间。没有经过升汞处理的根段菌丝团污染率最大,而处理2~3min后根段的菌丝团污染率最小。
译  名:
Study on Isolation and Culture Characteristics of Paphiopedilum micranthum Mycorrhizal Fungi
作  者:
TIAN Fan1,ZHU Guo-sheng2,3,GUI Yang2,3,BAI Xin-xiang1 (1.Guizhou University,Guiyang,Guizhou 550025;2.Institute of Morden Chinese Medical Materials,Guizhou Academy of Agricaltural Sciences,Guiyang,Guizhou 550006;3.Guizhou Key Laboratory of Agricultural Biotechnology,Guiyang,Guizhou 550006)
关键词:
Paphiopedilum micranthum;mycorrhizal fungi;peloton;isolation;culture
摘  要:
Eight mycorrhizal endophyte strains were isolated from a single peloton of Paphiopedilum micranthum.After purification,the strains were cultured by the hole method and the morphological characteristics were observed.At the same time,the growth rate was determinated.Isolated strains were named as DJYYDL001,DJYYDL003,DJYYDL004,DJYYDL006,DJYYDL008,DJYYDL009,DJYYDL011,DJYYDL015.The results showed that the single sclerotium isolated from the pelotons were cultured in thermostatic,after 12 hours,microscopy and absorb the hyphae grow group was the best time.Without mercuric chloride treatment root sclerotium pollution rate,while processing 2~3 min posterior root segment sclerotium contamination rate minimum.

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