中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 硬叶兜兰菌根真菌的分类鉴定 北方园艺 2017 (24) 116-122
Position: Home > Articles > Classification and Identifition of Mycorrhizal Fungi of Paphiopedilum micranthum Northern Horticulture 2017 (24) 116-122

硬叶兜兰菌根真菌的分类鉴定

作  者：
田凡;廖小锋;王莲辉;朱国胜;桂阳;白新祥
关键词：
硬叶兜兰;菌根真菌;丝核菌;序列分析;鉴定
摘  要：
以硬叶兜兰菌根真菌为研究对象,采用传统形态学结合rDNA-ITS区的PCR扩增、测序,采用NCBI BLAST在线软件对ITS区序列进行了分析,对硬叶兜兰菌根真菌进行分类鉴定。结果表明:对29株菌根真菌进行rDNA-ITS序列分析共鉴定出23个属,为瘤菌根菌属、胶膜菌属、丝核菌属,镰刀菌属、栓菌属、附毛菌属、拟迷孔菌属、小脆柄菇属、拟层孔菌属、毛壳属、侧耳属、轮枝菌属、弯孢聚壳属等,分离菌株最多的是瘤菌根菌属和胶膜菌属,分别占分离总数的16%和19%;鉴定到1个科,为胶膜菌科;12株优势菌根具有不同的宏观和微观形态,其均未有分生孢子,菌丝分枝点附近形成一个隔及细胞双核,11株菌株具念珠状细胞,大多数具有香味。
译  名：
Classification and Identifition of Mycorrhizal Fungi of Paphiopedilum micranthum
作  者：
TIAN Fan;LIAO Xiaofeng;WANG Lianhui;ZHU Guosheng;GUI Yang;BAI Xinxiang;Guizhou Forestry Academy;Institute of Mountain Resources,Guizhou Academy of Sciences;Institute of Morden Chinese Medical Materials,Guizhou Academy of Agricaltural Sciences;College of Forestry,Guizhou University;
单  位：
Guizhou Forestry Academy%Institute of Mountain Resources,Guizhou Academy of Sciences%Institute of Morden Chinese Medical Materials,Guizhou Academy of Agricaltural Sciences%College of Forestry,Guizhou University
关键词：
Paphiopedilum micranthum;;mycorrhizal fungi;;Rhizoctonia;;rDNA ITS analysis;;identifition
摘  要：
Classification and identification of mycorrhizal were associated with Paphiopedilum micranthum,the method of morphological was combined with the ITS gene fragments were PCR amplified and sequenced,the rDNA ITS regions were analyzed by means of the software of NCBI BLAST.The results showed that the 29 strains with different micro-and macro-morphological.Secondly,rDNA-ITS sequence analysis of 29 predo minant strains.The fungi were identified into 23 genus and 1 family,23 strains belong to Epulorhiza,Tulasnella,Rhizoctonia,Fusarium,Trametes,Trichaptum, Daedaleopsis, Psathyrella, Fomitopsi, Chaetomium, Pleurotus, Lecanicillium,Eutypella,Lecanicillium,Peniophorella,which their frequency were 16% and 19%.Only one family belonged to Tulasnellaceae.12 strains of dominant mycorrhiza had different microcosmic and macroscopic,none of them had conidiophore,near the hyphal branching-points formed of septum and conjugate nuclei,11 strains mycorrhiza with intestiniform cells,the most of mycorrhiza had a distinct aroma.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46