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Position: Home > Articles > Aseptic Sowing and in vitro Seedling Culture of Paphiopedilum micranthum T.Tang & F.T.Wang Northern Horticulture 2011 (5) 115-117

硬叶兜兰的无菌播种和试管成苗

作  者:
丁长春;李蕾;夏念和
单  位:
文山学院生化系;中国科学院研究生院;中国科学院华南植物园
关键词:
硬叶兜兰;无菌播种;试管成苗
摘  要:
以硬叶兜兰的种子为试材,研究种子成熟度、种子预处理以及培养基成分对种子萌发、原球茎分化以及试管成苗的影响。结果表明:果龄110~210 d的种子经处理后均能够萌发,其中150 d的萌发率最高,达40%。10%的84消毒液预处理对种子萌发有显著促进作用,在5~20 min范围内,以11 min处理获得最佳萌发效果;处理时间20 min时萌发率最低,为11%。1/5MS基本培养基较花宝培养基有更高的萌发率,添加AgNO31 mg/L利于原球茎分化及试管苗的发育。
译  名:
Aseptic Sowing and in vitro Seedling Culture of Paphiopedilum micranthum T.Tang & F.T.Wang
作  者:
DING Chang-chun1,2,LI Lei3,XIA Nian-he1(1.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650;2.Graduate University of Chinese Academy of Sciences,Beijing 100049;3.Department of Biology and Chemistry,Wenshan College,Wenshan,Yunnan 663000)
关键词:
Paphiopedilum micranthum T.Tang & F.T.Wang;seed germination;seedling growth
摘  要:
In the report,we studied the effects of seed maturity,seed pretreatments and media component on the asymbiotic germination of Paphiopedilum micrahthum.The results showed that the timing of seed collection was critical in maximizing germination percentage in this species.The seeds collected from 110 to 210 days after pollination(DAP) was capable for germination,the optimum for culture in vitro was 150 days.Pretreatment of the mature seeds with 10% NaOCl for 11 min were optimum for improving the germination percentage.Differentiation of protocorm and further seedling growth was promoted by AgNO3(1.0 mg/L).

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