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Position: Home > Articles > Analysis of Polypeptides of Rabbit Haemorrhagic Disease Virus by Isoelectric Focusing Acta Agriculturae Universitatis Jiangxiensis 1999 (3) 404-406

等电聚焦分析兔出血症病毒多肽

作  者:
王恒安;杜念兴;徐文燕
单  位:
南京农业大学;上海农学院动物科学系
关键词:
兔出血症病毒;等电聚焦
摘  要:
人工感染 N J 株 R H D V 病死兔肝经低温反复冻融、氯仿处理、 P E G 沉淀、 Sepharose4 B 柱层析得纯化病毒。纯化病毒经尿素- 聚丙烯酰胺圆盘等电聚焦,考马斯亮蓝染色后紫外扫描显示8 条多肽,其等电点及相对百分含量 分别为 A:6 .3 ,18 .51 % ; B:6 .7 ,11 .22 % ; C:7 .4 ,4 .90 % ; D:7 .7 ,8 .53 % ; E:8 .1 ,7 .27 % ; F:8 .7 ,12 .44 % ; G:9 .0 ,16 .18 % ; H:9 .5 ,22 .27 % .说明 R H D V 蛋白主要由碱性蛋白组成。
译  名:
Analysis of Polypeptides of Rabbit Haemorrhagic Disease Virus by Isoelectric Focusing
作  者:
Wang Hengan 1 Du Nianxing 2 Xu Wenyan 2 (1Dept of Animal Science, Shanghai Agriculture College, Shanghai 201101;2Nanjing Agriculture University, Nanjing210095)
关键词:
rabbit haemorrhagic disease virus; isoelectric focusing
摘  要:
Rabbit haemorrhagic disease virus (RHDV) were purified from the infected liver emulsion by freezing and thawing, chioroform extraction, PEG precipitation and Sepharose-4B Chromatography. Eight peptides were scanned by uliraviolet (560nm) after isoelectric focusing the isoelectric point and the relative quantity of each peptide are A:6.3, 18.50%;B:6.7, 11.22%;C:7.4, 4.90%;D:7.7, 8.53%;E:8.1, 7.27%;F:8.7, 12.44%;G:9.0, 16.18%;H:9.5, 22.27% respectively. The results showed the proteins of RHDV are mainly composed of basic proteins.

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