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Position: Home > Articles > Identification of a Penicillium expansum Strain Producing Citrinin and Optimization of Culture Conditions for Citrinin Production FOOD SCIENCE 2018 (24) 162-167

1株产桔青霉素扩展青霉的鉴定及其产毒条件优化

作  者：
李慧芳;赵利娜;郑香峰;王芸;刁军委;张晓云;张红印
单  位：
江苏大学生命科学研究院;江苏大学食品与生物工程学院
关键词：
扩展青霉;桔青霉素;菌丝生物量;CIT产量
摘  要：
在腐烂柑橘表面筛选出1株产桔青霉素(citrinin,CIT)的菌株H1,经鉴定为扩展青霉,并进一步研究培养基、温度、pH值、溶氧量、培养时间等对菌株H1生长及产CIT能力的影响。结果表明:在pH 3的YSM培养基上25℃摇床培养2周,菌株产生的CIT产量最高,为12.8μg/g,pH 11的碱性条件下产毒受到抑制。
译  名：
Identification of a Penicillium expansum Strain Producing Citrinin and Optimization of Culture Conditions for Citrinin Production
作  者：
LI Huifang;ZHAO Lina;ZHENG Xiangfeng;WANG Yun;DIAO Junwei;ZHANG Xiaoyun;ZHANG Hongyin;College of Food Science and Biological Engineering, Jiangsu University;Institute of Life Sciences, Jiangsu University;
单  位：
LI Huifang%ZHAO Lina%ZHENG Xiangfeng%WANG Yun%DIAO Junwei%ZHANG Xiaoyun%ZHANG Hongyin%College of Food Science and Biological Engineering, Jiangsu University%Institute of Life Sciences, Jiangsu University
关键词：
Penicillium expansum;;citrinin;;mycelial biomass;;CIT production
摘  要：
In this study, a strain named H1 capable of producing citrinin(CIT) was isolated from rotten citrus and identified as Penicillium expansum. The effects of different culture media, temperature, pH value, culture time and dissolved oxygen on the mycelial growth and CIT production of strain H1 were explored. The results showed that when P. expansum H1 was cultured in yeast extract-sucrose liquid medium with an initial pH of 3 at 25 ℃ with shaking for 2 weeks, the CIT content reached the highest value of 12.8 μg/g, and the CIT production was inhibited at pH 11.

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