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Position: Home > Articles > Screening and phylogenetic analysis of nitrate-reducing bacteria in salted fish Science and Technology of Food Industry 2011 (8) 213-215

腌鱼中硝酸盐还原菌的筛选及系统发育分析

作  者:
何燕飞;李和生;董亚辉;史秉鑫;彭娴
单  位:
宁波大学生命科学与生物工程学院
关键词:
腌鱼;硝酸盐还原菌;16SrDNA;系统发育分析
摘  要:
微生物作用是硝酸盐还原成亚硝酸盐的主要原因,而亚硝酸盐在一定条件下会合成强致癌物质——亚硝胺类化合物,存在潜在的食品安全问题。从浙江省特色腌制水产品腌鱼中分离形态颜色各异的菌株,应用硝酸盐还原实验快速筛选具有硝酸盐还原能力的菌株,进而对筛选的菌株进行形态观察、革兰氏染色、生理生化与分子生物学鉴定。最终筛选出有两株菌具有硝酸盐还原性,F-1菌株为Psychrobacter glacincola,F-2菌株为Psychrobacter faecalis。
译  名:
Screening and phylogenetic analysis of nitrate-reducing bacteria in salted fish
作  者:
HE Yan-fei,LI He-sheng*,DONG Ya-hui,SHI Bing-xin,PENG Xian(College of Life Science and Bioengineering,Ningbo University,Ningbo 315211,China)
关键词:
salted fish;nitrate-reducing bacteria;16S rDNA;phylogenetic analysis
摘  要:
The microbial reduction is the main reason of transferring nitrate into nitrite.But under certain conditions,nitrite would be synthesized in the strong carcinogens-nitrosamines compound which is a potential food safety problem.Bacterial strains in different color and form were isolated from the salted fish in Zhejiang,and nitrate reducing strains were screened by the nitrate reduction test according to the conversion ability of transferring nitrate to nitrite,then identified by the morphological,gram stain,physiological-biochemical and molecular biological identification.Two nitrate-reducing bacteria was screened,F-1 was Psychrobacter glacincola and F-2 was Psychrobacter faecalis.

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