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当前位置: 首页 > 文章 > Effect of Semen vaccariae and Taraxacu mogono on Cell Adhesion of Bovine Mammary Epithelial Cells 农业科学学报 (英文) 2012,11 (12)
Position: Home > Articles > Effect of Semen vaccariae and Taraxacu mogono on Cell Adhesion of Bovine Mammary Epithelial Cells Journal of Integrative Agriculture 2012,11 (12)

Effect of Semen vaccariae and Taraxacu mogono on Cell Adhesion of Bovine Mammary Epithelial Cells

作  者：
Tong Jin-jin;Li Ye;Liu Rong;Gao Xue-jun;Li Qing-zhang
单  位：
NE Agr Univ, Minist Educ, Key Lab Dairy Sci, Coll Life Sci, Harbin 150030, Peoples R China
关键词：
E-cadherin;beta-catenin;Semen vaccariae;Taraxacu mogono;bovine;mammary epithelial cells
摘  要：
The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and beta-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, beta-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher (P<0.05) in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly (P<0.05). In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, beta-catenin and CycinD1 in the Wnt signaling pathway.

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