中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 蚊虫乙酰胆碱酯酶的真核表达、纯化及活性测定 华中农业大学学报 2012,31 (4) 423-427
Position: Home > Articles > Expression and purification of acetylcholinesterase from culex by Bac-to-Bac baculovirus system and the activity determination Journal of Huazhong Agricultural University 2012,31 (4) 423-427

蚊虫乙酰胆碱酯酶的真核表达、纯化及活性测定

作  者：
谭烽;兰文升;崔峰;乔传令;陈雯莉
单  位：
中国科学院动物研究所农业虫害鼠害综合治理国家重点实验室;华中农业大学农业微生物学国家重点实验室
关键词：
有机磷农药;乙酰胆碱酯酶;昆虫杆状病毒表达系统;纯化酶;生物传感器
摘  要：
利用Trizol法从尖音库蚊中提取总RNA,构建cDNA文库,并克隆出乙酰胆碱酯酶外显子序列;利用Bac-to-Bac杆状病毒表达系统对蚊虫乙酰胆碱酯酶进行真核表达,并利用Ni-琼脂糖对酶进行纯化。采用SDS-PAGE对纯化产物进行检测,结果表明得到了纯度较高的乙酰胆碱酯酶。参照Ellman法对酶的活性进行测定,结果显示纯酶的活力为2.219×10-4 mol/(min.g)。
译  名：
Expression and purification of acetylcholinesterase from culex by Bac-to-Bac baculovirus system and the activity determination
作  者：
TAN Feng1,2 LAN Wen-sheng2 CUI Feng2 QIAO Chuan-ling2 CHEN Wen-li1 1.State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University,Wuhan 430070,China; 2.State Key Laboratory of Integrated Management of Pest Insects & Rodents in Agriculture, Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China
关键词：
organic phosphorus pesticides;acetylcholinesterase;baculovirus expression system;purified enzyme;biosensor
摘  要：
The residues of the high toxic organophosphorous can cause chronic poisoning.At present,the detection of organophosphorous residue needs complicated operation,time consuming and high cost.Here,we harvested total RNA of mosquito by Trizol methods,constructed cDNA library,and got the exon of acetylcholinesterase.The acetylcholinesterase from culex was expressed by Bac-to-Bac system,then the enzyme was purified using Ni-sepharose.The crude and purified enzyme was tested and verified by SDS-PAGE.The enzyme activity was determined by Ellman methods.It will be of great significance to biosensors in organophosphorous pesticides detection.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46