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Position: Home > Articles > Cloning and sequencing of a tick salivary function gene in Hyalomma asiaticum Animal Husbandry & Veterinary Medicine 2005,37 (5) 4-6

亚洲璃眼蜱唾液腺一新功能基因的克隆与测序

作  者:
程天印;周金林;施启顺;周勇志;林矫矫
单  位:
中国农科院上海家畜寄生病研究所;湖南农业大学动科院
关键词:
亚洲璃眼蜱;3-′RACE;HaB1
摘  要:
HaB1是亚洲璃眼蜱雌成蜱唾液腺差异表达基因文库中的一个片段,根据其序列设计引物HaB1-GSP1和HaB1-GSP2,以唾液腺总RNA为模板,RACE法扩增获得HaB1的未知3′-末端。测定该末端序列,进行序列拼接,设计全长引物5-′CCAGTCCGAAGGAAGGGCG-3′和5-′TCTC-CGGGCACGTGAAGTGTC-3′。以cDNA第一链为模板,扩增获得基因全长。经核查表明,该基因为一新基因(登录号AY803896)。
译  名:
Cloning and sequencing of a tick salivary function gene in Hyalomma asiaticum
作  者:
CHENG Tian-yin,ZHOU Jin-lin,SHI Qi-shun,ZHOU Yong-zhi,LIN Jiao-jiao(1.Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Science, Shanghai 200232,China;2.College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128,China)
关键词:
Hyalomma asiaticum;3-RACE;HaB1
摘  要:
HaB1 is a cDNA fragment from the libraries of differentially expressed genes in the salivary glands of the partially engorged Hyalomma asiaticum female tick. Based on its sequence, a pair of primers, HaB1-GSP_1 and HaB1-GSP_2, were designed. The 3 end was obtained from total RNA of the salivary gland in H.asiaticum with RACE, and then a new gene (AY803896) was cloned with 5-CCAGTCCGAAGGAAGGGCG-3 and 5-TCTCCGGGCACGTGAAGTGTC-3 as primers.

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