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Position: Home > Articles > Species identification of an imported tick sample of Hyalomma asiaticum asiaticum by genetic analysis at Alashankou port,China-Kazakhstan border Chinese Journal of Veterinary Medicine 2016,52 (11) 17-19

中哈边境阿拉山口口岸输入性亚洲璃眼蜱的种类鉴定与分析

作  者:
徐军;王安东;戴莉;杨杰;罗丹;徐建军;李志远;热依罕古丽;萨尼叶古丽
单  位:
石河子大学医学院;石河子大学动物科技学院;阿拉山口出入境检验检疫局;新疆出入境检验检疫局;新疆农业大学动物医学学院
关键词:
亚洲璃眼蜱;种类鉴定;16S RNA;COI;线粒体;阿拉山口口岸
摘  要:
对1批从哈萨克斯坦进口羊毛中检获的蜱样品进行种类鉴定。应用16S rDNA基因和COI基因两条DNA条形码技术进行鉴定分析。结果 16S rDNA序列分析显示与新疆亚洲璃眼蜱(KC203351)同源性最接近(99%);COI基因序列分析显示与新疆伊犁亚洲璃眼蜱(KF527440)同源性最接近(99%)。结论:经DNA条形码比对,确定为亚洲璃眼蜱,应加强对外来医学媒介生物防控,严防"外来病"入侵。
译  名:
Species identification of an imported tick sample of Hyalomma asiaticum asiaticum by genetic analysis at Alashankou port,China-Kazakhstan border
作  者:
XU Jun;WANG An-dong;DAI Li;YANG Jie;LUO Dan;XU Jian-jun;LI Zhi-yuan;RE Yi-han-gu-li;SA Ni-ye-gu-li;Xinjiang Entry-Exit Inspection and Quarantine Authority of the PRC;College of Animal Science and Technology,Shihezi University;Alashankou Entry-Exit Inspection and Quarantine Authority of the PRC;College of Veterinary Medicine,Shihezi University;College of Veterinary Medicine,XND;
关键词:
Hyalomma asiaticum asiaticuma;;Speciesi dentification;;16 S rDNA;;COI gene;;Alashankou Port;;China-Kazakhstan border
摘  要:
To identify the species of ticks in wool samples from Kazakhstan at Alashankou port,Xinjiang Uygur Autonomous Region,China.The 16 S rDNA and CO1 identification were carried out for the ticks,and sequence analysis was performed to establish the molecular phylogenetic tree.The 16 S rDNA and COI were clustered together with Hyalomma asiaticum asiaticum(accessionnumbers:KC203351,KF527440);these strains had the highest homology with Hyalomma asiaticum asiaticum(99 %).Based on DNA barcode comparison,the imported tick sample was identified as Hyalomma asiaticum asiaticum.We should strengthen the prevention and control of imported medical vectors,to prevent the " Exotic Diseases".

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