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当前位置: 首页 > 文章 > 心肌细胞特异性BRCC36转基因小鼠的构建 扬州大学学报(农业与生命科学版) 2018 (2) 53-58+79
Position: Home > Articles > Construction of transgenic mice expressing BRCC36 in cardiomyocyte Journal of Yangzhou University(Agricultural and Life Science Edition) 2018 (2) 53-58+79

心肌细胞特异性BRCC36转基因小鼠的构建

作  者：
姚德山;郑海霞;朱华江;沈慧;张丽娜;张振刚;龚开政
关键词：
Gateway技术;去泛素化酶BRCC36;心肌细胞;特异性表达;转基因小鼠
摘  要：
为构建心肌细胞过表达BRCC36转基因小鼠,利用Gateway技术原理构建BRCC36过表达载体,用显微注射技术将构建的载体注入小鼠受精卵中,转移到代孕母鼠内自然生产;通过PCR法鉴定筛选阳性子代转基因小鼠;提取阳性转基因小鼠心脏组织蛋白,通过Western blot方法检测BRCC36表达量;比较转基因阳性小鼠与野生型小鼠生长发育情况。结果表明:成功地将外源性BRCC36基因整合到子代转基因小鼠基因组中,并在SP级环境下饲养繁殖到10代以上;转基因阳性小鼠心脏组织中BRCC36表达均明显增高且稳定表达,阳性转基因小鼠与野生型小鼠生长发育没有明显差异。这一研究成功构建了心肌细胞特异性过表达BRCC36转基因小鼠,为进一步研究BRCC36在病理性心肌重构中的作用提供了理想的工具小鼠。
译  名：
Construction of transgenic mice expressing BRCC36 in cardiomyocyte
作  者：
YAO Deshan;ZHENG Haixia;ZHU Huajiang;SHEN Hui;ZHANG Li'na;ZHANG Zhengang;GONG Kaizheng;The Affliated Hospital/Cardiovascular Institute,Yangzhou University;
单  位：
YAO Deshan%ZHENG Haixia%ZHU Huajiang%SHEN Hui%ZHANG Li'na%ZHANG Zhengang%GONG Kaizheng%The Affliated Hospital/Cardiovascular Institute,Yangzhou University
关键词：
Gateway technology;;deubiquitination enzyme BRCC36;;cardiomyocyte;;specific expression;;transgenic mice
摘  要：
BRCC36 overexpression vector was constructed by Gateway technology.The vector was injected into fertilized ovum by microinjection technology,then transferred to the pseudopregnant mice and waited for eutocia.PCR was performed to identify the positive BRCC36 transgenic mice.Protein was extracted from the cardiac tissue of positive transgenic mice and the expression of BRCC36 was detected by Western blot.The growth and development were compared in the positive and WT mice.Objective of the study was to establish a stably overexpressing BRCC36 transgenic mouse and to provide qualified tool mice for studying the role of BRCC36 in pathological myocardial remodeling.Results showed that exogenous BRCC36 gene was steadily integrated into the genome of the progeny transgenic mice and bred more than 10 generations in SPF environment.The expression of BRCC36 in the cardiac tissue of transgenic mice was obviously increased and expressed steadily.There was no significant difference in the growth and development between the positive transgenic mice and the wild type mice.The cardiomyocyte specific overexpression of BRCC36 transgenic mice are constructed.This transgenic mice can be a good tool for further study of the role of BRCC36 in pathological myocardial remodeling.

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