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当前位置: 首页 > 文章 > 黄姜中自身转化薯蓣皂苷元提取的研究 安徽农业科学 2010,38 (3) 1616-1617+1645
Position: Home > Articles > Research on Extraction of Diosgenin through Itself Transformation in Dioscorea zingiberensis C.H.Wright Journal of Anhui Agricultural Sciences 2010,38 (3) 1616-1617+1645

黄姜中自身转化薯蓣皂苷元提取的研究

作  者：
王颖;富瑶瑶;刘廷强;鱼红闪;金凤燮
单  位：
大连工业大学生物与食品工程学院
关键词：
薯蓣皂苷元;大孔吸附树脂;高效液相色谱
摘  要：
[目的]研究黄姜中自身转化薯蓣皂苷元的提取方法。[方法]基于黄姜中自身酶的作用,将黄姜在室温条件下分别保存15和30d,使带有3个糖基的薯蓣皂苷在其自身酶作用下部分转化生成无糖基薯蓣皂苷元,并对黄姜自身转化薯蓣皂苷元进行提取、分离、检测,比较不同保存时间的提取效果。[结果]500 g黄姜在室温下保存15和30 d,经70%乙醇浸提分别得薯蓣总皂苷30.8和24.7 g,总皂苷得率分别为6.16%和4.94%,用大孔吸附树脂AB-8分离提纯后分别得薯蓣皂苷元6.3和11.7 g,苷元得率分别为1.26%和2.34%,继后利用D-280大孔吸附树脂脱色最终得薯蓣皂苷元分别为4.97和10.2 g,得率分别为0.99%和2.04%。[结论]保存30 d的黄姜,经大孔吸附树脂分离纯化后,其薯蓣皂苷元是保存15 d黄姜的2倍,经HPLC检测,其纯度为91%。
译  名：
Research on Extraction of Diosgenin through Itself Transformation in Dioscorea zingiberensis C.H.Wright
作  者：
WANG Ying et al(College of Biology and Food Engineering,Dalian Industry University,Dalian,Liaoning 116034)
关键词：
Diosgenin;Macroporous absorption resin;HPLC
摘  要：
[Objective] The aim was to research the extraction method of diosgenin through itself transforming in Dioscorea zingiberensis C.H.Wright.[Method] D.zingiberensis was preserved for 15 and 30 d resp.under the room temperature condition on base of the effect of itself enzyme in D.zingiberensis And then the diosgenin with 3 glycosyl was part transformed into non-glycosyl diosgenin under the effect of itself enzyme,the itself transformed diosgenin in D.zingiberensis was extracted,separated and measured and the extraction effect under the different preserve times were compared.[Result] When 500g D.Zingiberensis were preserved for 15 and 30 d under the room temperature resp.,the diosgenin were got by 30.8 and 24.7 g resp.and the total saponin yield was 6.16 % and 4.94 % resp.through extracting by 70% ethanol;the diosgenin was got by 6.3 and 11.7 g resp.and the saponin yield was 1.26% and 2.34% after separation and purification by using macroporous absorption resin;and then the final diosgenin was 4.97 and 10.2g and its yield was 0.99% and 2.04% after decoloration by using D-280 macroporous absorption resin.[Conclusion] The diogenin in D.zingiberensis preserved at 30 d was 2 times of that preserved at 15 d after separation and purification by using macroporous absorption resin,and its purity was 91% by HPLC detection.

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