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Position: Home > Articles > Effect of Diosgenin on Proliferation and Cycle of HL-60 Cells Journal of Jilin Agricultural University 2003,25 (5) 61-63

薯蓣皂苷元对HL-60细胞增殖及细胞周期的影响

作  者:
李晶华;历艳志;张沐新;宋宇;王本祥;周秋丽
单  位:
吉林天药科技股份有限公司;吉林农业大学农学院;吉林大学再生医学科学研究所;吉林大学公共卫生学院;吉林大学药学院
关键词:
薯蓣皂苷元;HL-60细胞;细胞周期
摘  要:
采用XTT比色法和流式细胞仪技术观察薯蓣皂苷元(Diosgenin,Dio)对人急性髓性白血病细胞株HL-60的增殖抑制作用以及细胞周期变化。结果表明:XTT法检测其24h的半数抑制浓度(IC_(50))为7.36mg/L;流式细胞仪检测Dio作用24h后,G_0/G_1期细胞增多,S期细胞减少,凋亡细胞增多。Dio可明显抑制HL-60细胞的增殖。
译  名:
Effect of Diosgenin on Proliferation and Cycle of HL-60 Cells
作  者:
LI Jing-hua~1, LI Yan-zhi~2, ZHANG Mu-xin~3, SONG Yu~4, WANG Ben-xiang~4, ZHOU Qiu-li~5 (1. College of Public Health, Jilin University, Changchun, Jilin 130021, China; 2. College of Agronomy, Jilin Agricultural University, Changchun, Jilin 1300118, China; 3. College of Pharmacy, Jilin University, Changehun, Jilin 130021, China; 4. Jilin Tianyao Science and Technology Co. Ltd., Changchun, Jilin 130012, China; 5. Institute of Frontier Medical Science, Jilin University, Changchun, Jilin 130021, China)
关键词:
diosgenin; cell line HL- 60; cell cycle
摘  要:
The effect of diosgenin on cell diversion and cell cycle of human promyelocytic leukemia cell line HL-60 was determined by XTT assay and flow cytometer. The results showed that the IC_(50) was 7.36 mg/L after being incubated with diosgenin for 24h. The exposure of cells to diosgenin resulted in an increase of G_0/G_1-phase cells, a decrease of S-phase cells, and an increase of apoptotic cells. Diosgenin has an obvious inhibitory effect on cell proliferation.

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