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西藏绒山羊WNT4和HOXC13基因对绒毛纤维直径性状的遗传效应分析

作  者:
吴玉江;付雪峰;索朗达;巴贵;德吉;次仁德吉;宋天增;田可川
单  位:
西藏自治区农牧科学院畜牧兽医研究所;新疆畜牧科学院畜牧研究所
关键词:
西藏绒山羊;WNT4基因;HOXC13基因;纤维直径;SNP;遗传效应
摘  要:
试验旨在探索WNT4和HOXC13基因多态性及其对西藏绒山羊绒毛纤维直径性状的影响,寻找与西藏绒山羊绒毛纤维直径性状相关的分子标记。以380只1岁西藏绒山羊群体为研究对象,利用混池DNA直接测序法检测WNT4和HOXC13基因的SNP,利用飞行时间质谱技术对SNP分型,利用SAS 9.1软件中最小二乘方差模型对SNP位点与绒毛平均纤维直径、纤维直径标准差、纤维直径变异系数进行关联分析。结果表明,WNT4基因第3外显子区域检测到2个SNPs位点(SNP1和SNP2),HOXC13基因第2外显子区域检测到2个SNPs位点(SNP3和SNP4),均处于中度多态(0.25
译  名:
Genetic Effects Analysis of WNT4 and HOXC13 genes on Fiber Diameter Trait in Tibetan Cashmere Goats
作  者:
WU Yujiang;FU Xuefeng;SUO Langda;BA Gui;DE Ji;CI Rendeji;SONG Tianzeng;TIAN Kechuan;Institute of Animal Science,Tibet Academy of Agricultural and Animal Husbandry Sciences;Institute of Animal Science,Xinjiang Academy of Animal Sciences;
关键词:
Tibetan cashmere goats;;WNT4 gene;;HOXC13 gene;;fiber diameter;;SNP;;genetic effect
摘  要:
The aim of this study was to explore the effects of WNT4 and HOXC13 genes polymorphisms on the fiber diameter trait of Tibetan cashmere goats,in order to find out molecular markers related to fiber diameter trait.380 one year old Tibetan cashmere goats were selected as experimental animals.The single nucleotide polymorphism(SNP) of WNT4 and HOXC13 genes were detected by pooled DNA sequencing method,and genotyped by Sequenom MassARRAY genotype technology.The SNPs loci associations with cashmere mean fiber diameter(MFD),fiber diameter standard deviation(FDSD),coefficient of variation of fiber diameter(CVFD) traits were analyzed using the least squares method in the GLM procedure of SAS 9.1 software.The results showed that two SNPs(SNP1 and SNP2) were identified in exon 3 of WNT4 gene,and two SNPs(SNP3 and SNP4) were identified in exon 2 of HOXC13 gene,respectively,the genetic variation was moderate polymorphism(0.25

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