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Position: Home > Articles > Effects of Genotype Ⅳ HEV ORF3 on Expression of Adipokine in Target Cells Progress in Veterinary Medicine 2013,34 (6) 78-80

基因Ⅳ型戊型肝炎病毒ORF3对靶细胞脂肪因子表达的影响

作  者:
杜丽;成鹰;史巧芸;焦寒伟;荣辉;张珈宁;贾晓晓;郭莳雨;朱华培
单  位:
海南大学农学院;海口市动物基因工程重点实验室;海南省热带动物繁育与疫病研究重点实验室
关键词:
蛋白芯片;戊型肝炎病毒;靶细胞;脂肪因子;表达
摘  要:
为了探讨戊型肝炎病毒ORF3影响靶细胞脂肪因子表达的分子机制,应用蛋白芯片技术比较稳定表达EGFP-ORF3的HEK293细胞和稳定表达EGFP的HEK293细胞的脂肪因子表达谱,筛选获得显著上调/下调的脂肪因子。结果表明,在稳定表达EGFP-ORF3的HEK293细胞,Fas和TIMP-2的表达水平升高,而ACE-2表达水平降低。研究结果为HEV ORF3对于靶细胞重要脂肪因子表达影响的分子机制研究提供了重要依据。
译  名:
Effects of Genotype Ⅳ HEV ORF3 on Expression of Adipokine in Target Cells
作  者:
DU Li,CHENG Ying,SHI Qiao-yun,JIAO Han-wei,RONG Hui,ZHANG Jia-ning, JIA Xiao-xiao,GUO Shi-yu,ZHU Hua-pei,WANG Feng-yang(1.College of Agriculture,Hainan University,Haikou,Hainan,570228,China;2.Hainan Key Lab of Tropical Animal Reproduction &Breeding and Epidemic Disease Research,Haikou,Hainan,570228,China; 3.Animal Genetic Engineering Key Lab of Haikou City,Haikou,Hainan,570228,China)
关键词:
protein array;Hepatitis E virus;target cell;adipokine;expression
摘  要:
Human adipokine antibody array was used to compare the expression levels of adipokines in stable cell lines that expressed or did not express SHEV ORF3.The results indicated that the expression of Fas and TIMP2 in ORF3 expressing human embryonic kidney 293 cell line was upregulated,the expression of ACE-2 in ORF3 expressing human embryonic kidney 293 cell line was downregulated,which provide important information for elucidating the molecular mechanism through which HEVORF3 affects the expression of adipokine in target cells.

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