作 者：
陈景耀;张卫明;王习达;吴婷;陆长梅;吴国荣
单 位：
江苏省药用植物生物技术重点实验室;南京野生植物综合利用研究院;江苏省海洋环境监测中心;南京师范大学生命科学学院
摘 要：
在分离纯化白芨多糖并对其硫酸酯化的基础上,比较研究白芨多糖(Bletilla striata polysaccharide,BSPS)及其酯化产物白芨多糖硫酸酯(BSPSS)的部分理化性质及生物学活性。实验结果表明,BSPSS在水中的溶解度明显提高,分子量为9．0607×10~4,BSPS分子量为9．9658×10~4;酯化物硫酸基取代度为1．31,硫酸基接在C-6上。BSPSS在化学模拟系统中去除超氧阴离子自由基(O_2~-·)的作用显著增强,低浓度时也明显具有去除羟基自由基(·OH)的作用,各实验浓度的BSPS清除O_2~-·的作用不明显,而对清除·OH而言则随着浓度升高其清除作用也趋增强。一定浓度的BSPSS和BSPS均具有抑制H_2O_2诱导的红细胞溶血作用;白芨多糖的硫酸酯化有助于增强B淋巴细胞的增殖作用和对体外白血病细胞株U937生长的抑制作用。
译 名：
Comparision Study on Bioactivities of Bletilla striata(Thunb.)Reichb.f.Polysaccharide and BSPSS
作 者：
CHEN Jing-yao~(1.2) ZHANG Wei-ming~3 WANG Xi-da~4 WU Ting~2 LU Chang-mei~2 WU Guo-rong~(2,*) (1.Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province,Xuzhou 221116,China; 2.CoUege of Life Science,Nanjing Normal University,Nanjing 210097,China; 3.Nanjing Institute for Comprehensive Utilization of Wild Plant,Nanjing 210042,China; 4.The Ocean Environment Monitoring Center of Jiangsu Province,Nanjing 210036,China)
关键词：
Bletilla striata(Thunb.)Reichb.f.polysaccharide(BSPS);;sulfation;;bioactivitioes;;comparision
摘 要：
Bletilla striata polysaccharide(BSPS)was extracted,purified and sulfated.Some physical,chemical characterisitics and biological activities of BSPS and BSPSS(BSPS-sulfate)were studied in this paper.The solubility of BSPSS in water increases much more than that of BSPS.The mean molecular weight of BSPSS is 9.0607×10~4 and that of BSPS 9.9658×10~4.BSPSS has a stronger scavenging abifity on O_2~-·and·OH than that of BSPS.BSPSS and BSPS can inhibit the H_2O_2-induced hemolysis and promote proliferation of lymphocyte B.BSPSS has obvious inhibition on the growth of lymphocyte B and U937 in vitro.
