当前位置: 首页 > 文章 > HPLC法检测乳品中对羟基苯甲酸酯类和纳他霉素 乳业科学与技术 2013,36 (3) 9-11
Position: Home > Articles > Determination of Parabens and Natamycin in Dairy Products by HPLC Journal of Dairy Science and Technology 2013,36 (3) 9-11

HPLC法检测乳品中对羟基苯甲酸酯类和纳他霉素

作  者:
万鹏;赵贞;李翠枝;邵建波;王云霞;刘春霞
单  位:
内蒙古伊利实业集团股份有限公司
关键词:
液相色谱法;对羟基苯甲酸酯类;纳他霉素;乳制品
摘  要:
建立了乳制品中的对羟基苯甲酸酯类和纳他霉素的高效液相色谱分析方法。样品用甲醇提取后,使用C18反相色谱柱分离,以甲醇-醋酸水溶液为流动相,紫外检测器检测,检测波长256nm和305nm条件下5种防腐剂在35min之内完全分离。在一定条件下各组分的相关系数均大于0.9990,线形范围1~100mg/kg,回收率为83.7%~103%。
译  名:
Determination of Parabens and Natamycin in Dairy Products by HPLC
作  者:
WAN Peng,ZHAO Zhen,LI Cui-zhi*,SHAO Jian-bo,WANG Yun-xia,LIU Chun-xia(Inner Mongolia Yili Industrial Group Co.Ltd.,Hohhot 010010,China)
关键词:
HPLC;parabens;natamycin;dairy product
摘  要:
A method for determining parabens and natamycin in dairy products by HPLC is described.Samples were extracted with methanol and separated on a C18 column with methanol-acetic acid solution as mobile phase.UV detection was performed at 256 nm and 305 nm.Complete separation of pimaricin and four parabens,methyl 4-hydroxybenzoate,ethyl 4-hydroxybenzoate,propyl 4-hydroxybenzoate and butyl 4-hydroxybenzoate was achieved within 35 min.Under optimized conditions,the linear ranges for the food antiseptics was 1–100 mg/kg with a correlation coefficient greater than 0.9990,and their average recovery rates from blank real samples spiked at three different levels was between 83.7% and 103%.This method was simple,rapid and accurate.

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