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双酶法生产高纯度低聚果糖的研究

作  者:
LamiaL'Hocine;江波;王璋
单  位:
无锡轻工大学食品学院
关键词:
低聚果糖;果糖转移酶;葡萄糖氧化酶;过氧化氢酶
摘  要:
以果糖转移酶作用于蔗糖制得的低聚果糖为原料,将葡萄糖氧化酶及过氧化氢酶协同作用于30%的总糖,在30℃,pH5.0条件下,通气反应16h,得到纯度为86.92%(总低聚果糖占总糖)的低聚果糖。
关键词:
Fructo-oligosaccharides Fructosyltransferase Glucose oxidase catalase
摘  要:
High-purity of fructo-oligosaccharides (FOS) was prepared after glucose was removed in normal FOS products by glucose oxidase (GOD) and catalase (CAT). The optimum temperature and pH of glucose oxidase were 45℃and 5.0. respectively. 86.92 % of FOS was prepared by G0D (50U/g total sugar) and CAT (725U/g total sugar) at 30℃. pH 5.0, 3O% of total sugar concentration for 16 h aerated incubation.

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