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Position: Home > Articles > Fractionation of Polysaccharides from Chicken Keel Cartilage and Their Free Radical Scavenging Activities FOOD SCIENCE 2009,30 (5) 68-71

鸡胸软骨多糖组分的分级及自由基清除活性研究

作者：

熊双丽;张建国;袁小红;魏明

单位：

西南科技大学生命科学与工程学院

关键词：

鸡胸软骨;多糖;分级分离;自由基

摘要：

直接用木瓜蛋白酶水解鸡胸软骨,经三氯乙酸除蛋白质、乙醇沉淀、干燥得多糖粗品,采用DEAE-Sepharose FastFlow离子交换柱色谱和Sepharose 6B FastFlow分离纯化粗多糖,并进行光谱学分析和自由基清除活性研究。结果显示:乙醇沉淀多糖的自由基清除活性显著高于初步透析后的粗多糖,纯化后的硫酸软骨素的活性最小。粗多糖经DEAE-Sepharose FastFlow离子交换柱层析和Sepharose 6B FastFlow凝胶柱层析后,得到硫酸软骨素和其他5种非糖胺聚糖类多糖。5种多糖的自由基清除活性均显著大于硫酸软骨素。

译名：

Fractionation of Polysaccharides from Chicken Keel Cartilage and Their Free Radical Scavenging Activities

作者：

XIONG Shuang-li,ZHANG Jian-guo,YUAN Xiao-hong,WEI Ming (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

关键词：

chicken keel cartilage;polysaccharide;fractionation;free radical

摘要：

Crude polysaccharides were extracted from chicken keel cartilage by papain enzymolysis, deproteination of trichloracetic acid and ethanol precipitation, and then they were further purified by DEAE-Sepharose Fast Flow ion exchange column chromatography and Sepharose 6B Fast Flow gel column chromatography. Finally the scavenging activities of various fractions to hydroxyl radicals were compared. The results exhibited that the activity of crude polysaccharides precipitated by alcohol is significant higher than that of crude polysaccharides after preliminary dialysis. In addition, chondroitin sulfate and other 5 kinds of polysaccharides (non-glycosaminglycan) were obtained from the crude polysaccharides by DEAE-Sepharose Fast Flow ion exchange column chromatography and Sepharose 6B Fast Flow gel column chromatography. The scavenging activities of these 5 kinds of polysaccharides to hydroxyl radical are higher than that of chondroitin sulfate.

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鸡胸软骨多糖组分的分级及自由基清除活性研究

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