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Position: Home > Articles > A Study on the Preparation and Antioxidant Activity of Antioxidant Peptide from Mung Bean Acta Agriculturae Universitatis Jiangxiensis 2013,35 (5) 1063-1069

绿豆抗氧化活性肽的制备及其抗氧化活性研究

作  者:
李琴;张海生;许珊;赵换霞;孙键;张泽炎;张瑞妮
单  位:
陕西师范大学食品工程与营养科学学院
关键词:
绿豆蛋白;酶解;抗氧化肽;活性
摘  要:
研究了绿豆抗氧化活性肽的酶解制备工艺,并以Vc和BHT作为对照,对所制备的绿豆抗氧化活性肽的体外抗氧化活性进行了研究。结果表明:中性蛋白酶为制备绿豆抗氧化活性肽的理想蛋白酶,其最佳酶解工艺参数为:底物浓度2%,酶解pH 6.5,酶解温度50℃,加酶量5 000 u/g,酶解时间120 min。按此工艺条件制备的绿豆抗氧化活性肽具有较强的体外抗氧化活性,其抗氧化能力低于Vc,而高于BHT。
译  名:
A Study on the Preparation and Antioxidant Activity of Antioxidant Peptide from Mung Bean
作  者:
LI Qin;ZHANG Hai-sheng;XU Shan;ZHAO Huan-xia;SUN Jian;ZHANG Ze-yan;ZHANG Rui-ni;Food Engineering and Nutrition Science College,Shaanxi Normal University;
关键词:
mung bean;;enzymatic hydrolysis;;antioxidant peptide;;activity
摘  要:
The preparation of mung bean antioxidant peptide and its antioxidant capacity in vitro were studied and the results showed that neutral protease was the ideal protease for the preparation of mung bean antioxidant peptide.The best enzymatic hydrolysis technological parameters were as follows:substrate concentration 2%,hydrolysis pH 6.5,reaction temperature 50 ℃,enzyme concentration 5 000 u/g,reaction time 120 min.The mung bean antioxidant peptide prepared with these enzymatic hydrolysis technological parameters presented strong antioxidant activity and its antioxidant capacity was higher than that of BHT,but lower than that of Vc.

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