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花生红衣提取物中白藜芦醇、原花色素分离工艺的研究

作  者:
刘大川;刘强;吴波;徐金发
单  位:
武汉工业学院;山东青岛淳金植物油有限公司
关键词:
白藜芦醇;原花色素;大孔树脂;分离
摘  要:
对花生红衣提取物中白藜芦醇、原花色素的分离工艺进行了探讨。通过静态吸附、解吸实验选出AB-8大孔树脂,通过单因素、正交实验优化了白藜芦醇、原花色素的富集、分离条件。其吸附最佳条件为:上柱液流速1ml/min、pH7、白藜芦醇、原花色素的浓度浓度分别为2μg/ml和0.6mg/ml;解吸最适条件为:流速1.5ml/min、pH5;用20%甲醇溶液解吸其中的白藜芦醇,40%、60%甲醇溶液解吸原花色素,合并相同组分,经浓缩、冷冻干燥后制得纯度较高的白藜芦醇、原花色素产品,白藜芦醇的含量达43.6%,原花色素含量达93.05%。
译  名:
Research on Resin Separation Technology of Resveratrol and Procyanidin from Peanut Red Skin
作  者:
LIU Da-chuan1,LIU Qiang1,WU Bo1,XU Jin-fa2 (1.Wuhan Polytechnic University, Wuhan 430023, China; 2.Qingdao Chunjin Plant Oil Limited Company, Pingdu 266700, China)
关键词:
resveratrol;procyanidin;macroporous adsorption resin;separation
摘  要:
Research has been carried out on the technology of separating resveratrol and procyanidin extracted from the peanuts red skin. The macroporous adsorption resin AB-8 have been selected from 3 kinds of carriers by static adsorption and de-adsorption test. The single factor tests and orthogonal experiments have been applied to optimize the enrichment and separation condition of resveratrol and procyanidin. The optimum adsorption conditions were as follows: feeding rate 1ml/min, pH value 7.0 with the concentrations of resveratrol and procyanidin to be respectively 2μg/ml and 0.6mg/ml. The optimum elution conditions were elution rate 1.5ml/min and pH value 5.0. Resveratrol and procyanidin could be purified and separated by different concentration of methanol. Resveratrol was eluted by 20% methanol while procyanidin by 40% and 60%. After combining the same components, the products could be obtained by concentration and lyophilization with the contents of resveratrol and procyanidin as respectively 43.6% and 93.05%.

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