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Position: Home > Articles > Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken China Animal Husbandry & Veterinary Medicine 2015,42 (3) 525-530

猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立

作  者:
宋晓丽;刘梅轩;罗湘蜀;李成龙;杨术鹏;王战辉;张素霞
单  位:
中国农业大学动物医学院
关键词:
酶联免疫吸附法(ELISA);脱氧雪腐镰刀菌烯醇(DON);T-2毒素;猪肉;鸡肉
摘  要:
为保障动物源性食品安全,本研究建立了分别检测猪肉和鸡肉中脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和T-2毒素残留的间接竞争酶联免疫吸附法。结果显示,该方法在猪肉和鸡肉样本中DON的检测限分别为34.9和43.5μg/kg,添加回收率为72.7%~97.1%,变异系数小于8.7%;T-2毒素的检测限分别为33.7和28.7μg/kg,添加回收率为72.1%~95.0%,变异系数小于11.3%。该方法灵敏度高、准确、简便,适用于猪肉和鸡肉中DON和T-2毒素残留的快速检测。
译  名:
Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken
作  者:
SONG Xiao-li;LIU Mei-xuan;LUO Xiang-shu;LI Cheng-long;YANG Shu-peng;WANG Zhan-hui;ZHANG Su-xia;College of Veterinary Medicine,China Agricultural University;National Reference Laboratory for Veterinary Drug Residue;
关键词:
enzyme-linked immunosorbent assay(ELISA);;deoxynivalenol(DON);;T-2toxin;;pork;;chicken
摘  要:
To protect animal-origin foods,an indirect competitive enzyme-linked immunosorbent assay(icELISA)was developed to detect deoxynivalenol(DON)and T-2toxin in pork and chicken,respectively.The results showed that the limit of detection(LOD)of DON in pork and chicken were 34.9and 43.5μg/kg,respectively,and the recoveries ranged from 72.7%to 97.1% with the coefficients of variation(CV)lower than 8.7%.Besides,the LOD of T-2toxin in pork and chicken were 33.7and 28.7μg/kg,respectively,and the recoveries ranged from 72.1%to 95.0%with CV less than 11.3%.The developed method had the advantage of being sensitive and accurate,and was suitable for the rapid detection of DON and T-2residue in pork and chicken.

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