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禽肉中铜绿假单胞菌的分离及其耐药性

作  者:
魏超;代晓航;郭灵安;雷欣宇;刘月悦
单  位:
四川省农业科学院分析测试中心;农业部农产品质量安全风险评估实验室(成都)
关键词:
禽肉;铜绿假单胞菌;沙门氏菌;生化鉴定;基质辅助激光解吸电离飞行时间质谱
摘  要:
采用GB 4789.4—2010《食品安全国家标准食品微生物学检验沙门氏菌检验》中的选择性前增菌方法从鲜鹅肉、鸭肉样本中检测到2株疑似沙门氏菌的菌株,但经ATB生化鉴定和基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight-mass spectrometry,MALDI-TOF-MS)鉴定为铜绿假单胞菌。比对2株铜绿假单胞菌和沙门氏菌的生化图谱,其中鸟氨酸脱羧酶、精氨酸双水解酶和L-阿拉伯糖的实验结果同为阳性,其余生化反应结果差异较大;2株铜绿假单胞菌的质谱标识峰足迹与肠炎沙门氏菌CICC21482有明显不同。微生物耐药实验结果表明:大部分抗生素对铜绿假单胞菌无抑制作用,只有喹诺酮类抗生素环丙沙星、氨基糖苷类抗生素庆大霉素和链霉素对其有一定的抑制作用。
译  名:
Isolation and Drug Resistance of Pseudomonas aeruginosa from Poultry Meat
作  者:
WEI Chao;DAI Xiaohang;GUO Lingan;LEI Xinyu;LIU Yueyue;Analysis and Testing Center of Sichuan Academy of Agricultural Sciences;Agricultural Products Quality and Safety Risk Assessment Laboratory of Ministry of Agriculture (Chengdu);
关键词:
poultry meat;;Pseudomonas aeruginosa;;Salmonella;;biochemical identification;;matrix-assisted laser desorption-ionization time of flight mass spectrometry(MALDI-TOF-MS)
摘  要:
Two strains of suspected Salmonella spp were detected from fresh goose and duck samples by the selective pre-enrichment method according to the GB 4789.4-2010.However,the microorganism was identified as Pseudomonas aeruginosa by ATB biochemical identification and matrix-assisted laser desorption-ionization time of flight mass spectrometry(MALDI-TOF-MS).By analyzing the biochemical profiles of the two Pseudomonas aeruginosa strains and Salmonella spp,we found that the results of ornithine decarboxylase,arginine dihydrolase and L-arabinose tests were identically positive while the other biochemical results varied widely.Besides,the footprints of mass spectrometric peak of Pseudomonas aeruginosaand Salmonella enteritidis were significantly different.Drug resistance results showed that most of the antibiotics examined except ciprofloxacin,gentamicin and streptomycin could not inhibit Pseudomonas aeruginosa.

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