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25%吡唑·毒氟磷悬浮剂高效液相色谱分析方法研究

作  者:
卢瑞;陈小鹏;崔郭勤;王琳琳;韦小杰
单  位:
广西田园生化股份有限公司;广西大学化学化工学院
关键词:
毒氟磷;吡唑醚菌酯;悬浮剂;高效液相色谱
摘  要:
采用高效液相色谱法,以甲醇+水溶液为流动相、250mm×4.6mm(id)Agilent C_(18)色谱柱和紫外检测器,在250nm波长、柱温30℃和进样体积5μL条件下,对毒氟磷和吡唑醚菌酯进行定量分析。结果表明,毒氟磷的紫外吸收标准曲线方程为v=6 166 587.557 1x+79 698.995 7、吡唑醚菌酯的紫外吸收标准曲线方程为v=2 476 571.638 9x+26 998.154 9:毒氟磷和吡唑醚菌酯标准方程的线性相关系数分别为0.999 8和0.999 9,有效成分的平均值为20.41%和5.34%,标准偏差为0.09和0.03,变异系数为0.46%和0.55%,平均回收率为99.91%和100.21%。
译  名:
Study on HPLC Method Analyzing Pyraclostrobin and Dufulin 25% SC
作  者:
Lu Rui;Chen Xiaopeng;Wang Linlin;Wei Xiaojie;Cui Guoqin;School of Chemistry and Chemical Engineering,Guangxi University;Guangxi Tianyuan Biochemistry Co.,Ltd.;
关键词:
dufulin;;pyraclostrobin;;SC;;HPLC
摘  要:
An analytical method for the detection of Dufulin and Pyraclostrobin in Pyraclostrobin+Dufulin 25%suspension concentrates(SC) at once was established by high performance liquid chromatography(HPLC) with UV detector on 250 nm,250mm×4.6mm(id) Agilent C_(18) column at 30℃,using methanol aqueous solution as mobile phase and 5μL injection value.The results indicated that standard curve equationsof Dufulin and Pyraclostrobinwerey=6 166 587.557 1x+79 698.995 7 andy=2 476 571.638 9x+26 998.154 9,respectively,with the linear relationship coefficient of 0.999 8 and 0.999 9,the standard deviation of 0.09 and 0.03,the variation coefficient of 0.46%and 0.55%,and the average recovery of 99.91%and 100.21%,respectively.

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