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当前位置: 首页 > 文章 > 硫酸盐还原菌EMZY-1生理生化特性研究及16S rDNA序列分析 新疆农业科学 2008,45 (5) 801-804
Position: Home > Articles > Sequence Analysis on Physiologieal and Biochemical Characters of Sulfate Reducing Bacteria Strain EMZY-1 and 16s rDNA Xinjiang Agricultural Sciences 2008,45 (5) 801-804

硫酸盐还原菌EMZY-1生理生化特性研究及16S rDNA序列分析

作  者：
王沈海;罗淑萍;李佰广;李慧;董慧明;张颖
单  位：
大庆油田有限责任公司第二采油厂;中国科学院沈阳应用生态研究所;新疆农业大学农学院
关键词：
硫酸盐还原菌;分离;鉴定;16SrDNA
摘  要：
菌株EMZY-1是一株从中国大庆油田回注水中分离得到的硫酸盐还原菌(SRB)。通过对该菌株的形态、培养特征、生理生化特征的研究以及16S rDNA序列分析表明:该菌株为革兰氏阳性菌;细胞为棒杆状,端生孢子,大小约0.3×1.7(μm);温度低于10℃、高于60℃无明显生长;pH低于5.0、高于12.0无明显生长;NaCl质量浓度达到10%菌株不能生长;能在蔗糖、葡萄糖、甘露醇为C源的培养基上生长;NH4+、NO3-为菌株良好N源。菌株EMZY-1属于梭菌科。16S rDNA序列分析表明该菌与梭菌科中的Garciella nitratireducens菌同源性最高(99%),GenBank的注册号为EU275367,国内尚未见报道该类SRB。
译  名：
Sequence Analysis on Physiologieal and Biochemical Characters of Sulfate Reducing Bacteria Strain EMZY-1 and 16s rDNA
作  者：
WANG Shen-hai1,2,LUO Shu-ping1,LI Bai-guang3,LI Hui2,DONG Hui-ming2,ZHANG Ying2(1.College of Agronomy,Xinjiang Agricultural University,Urumqi,830052,China;2.Insititute of Applied Ecology.Chinese Academy of Sciences,Shenyang,110016,China;3.The Second Oil Production Plant,Daqing Oilfield Corporation Ltd.,Daqing,163414,China)
关键词：
sulfate-reducing bacteria;isolation;identification;16S rDNA
摘  要：
The strain EMZY-1,a Sulfate-Reducing Bacteria,was isolated from producing water in daqing oil field.The morphological analysis of strain EMZY-1 showed that it was a straight rod,about 0.3×1.7(μm) in size,and spherical and terminal spore appeared.Results of physiological and biochemical test showed that strain EMZY-1 was a Gram-positive bacteria,and could not grow when temperature was lower than 10℃ or higher than 60℃,pH was lower than 5.0 or higher than 12.0,and the concentration of NaCl was more than 10%.The strain EMZY could grow on the medium as a(resource)of sugar,glucose and mannite.The strain could use saccharose,glucose and mannitol as carbon resource,and NH4+ and NO3as nitrogen resource.The EMZY-1 belongs to clostridium family.The 16S rDNA sequence analysis indicated that the EMZY and the Garcialla nitratireducens have the highest homology(99%).The registered number in GenBank of the EMZY-1 was EU275367.It was a novel SRB that has never been reported before in China.

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