当前位置: 首页 > 文章 > 四氯化碳对厌氧降解系统中两类主要细菌的影响 农业环境科学学报 2007,26 (05) 1822-1826
Position: Home > Articles > Effect of Carbon Tetrachloride on Two Main Bacteria in Anaerobic Biodegradation System Journal of Agro-Environment Science 2007,26 (05) 1822-1826

四氯化碳对厌氧降解系统中两类主要细菌的影响

作  者:
单爱琴;王爱宽;韩宝平;杨秀婧;揣小明;张静
单  位:
中国矿业大学环境与测绘学院
关键词:
四氯化碳;厌氧系统;发酵性细菌;硫酸盐还原菌;毒性;生物降解
摘  要:
CCl4既作为电子受体,又是毒性极强的抑制剂,其生物毒性将影响微生物的活性、产物及生物降解的效率。采用多管发酵法(MPN),研究了初始四氯化碳浓度对厌氧系统中两类主要微生物——发酵性细菌和硫酸盐还原菌的毒性作用,为进行地下水及土壤厌氧生物修复提供理论依据。研究表明,四氯化碳浓度越高,对发酵性细菌和硫酸盐还原菌的毒性越强;且相同浓度的CCl4对硫酸盐还原菌的抑制作用较发酵性细菌要强得多;初始浓度(V/V)0.001%~0.005%的CCl4对发酵性细菌几乎没有抑制,而对硫酸盐还原菌总数的抑制率在47%~63%以上;浓度(V/V)高于0.01%的CCl4对发酵性细菌抑制作用较强,抑制率96%~99%,对硫酸盐还原菌的抑制作用更强,抑制率高达99.9%;在相同条件下,发酵性细菌比硫酸盐还原菌对四氯化碳的耐受性要强得多,微生物对四氯化碳的耐受程度将影响四氯化碳降解效率及降解产物。
译  名:
Effect of Carbon Tetrachloride on Two Main Bacteria in Anaerobic Biodegradation System
作  者:
SHAN Ai-qin,WANG Ai-kuan,HAN Bao-ping,YANG Xiu-jing,CHUAI Xiao-ming,ZHANG Jing (College of Environment & Spatial Informatics,China University of Mining and Technology,Xuzhou 221008,China)
关键词:
carbon tetrachloride; anaerobic system; fermentative bacterium; sulfate reducing bacterium; toxicity; biodegradation
摘  要:
Carbon tetrachloride served as electron donor and a serious inhibitor to life. The toxicity may impact the activity of microbes,the degradation rate and the biotransformation products of CCl4. Using method of most-possible-number (MPN),this research investigated the effects of initial CC14 concentrations on the two main microbes: fermentative bacterium (FB) and sulfate reducing bacterium (SRB) in anaerobic systems. This study may provide important evidence for anaerobic bioremediation of groundwater and soil contamination. The results indicated that increasing initial concentration of CC14 resulted in higher toxicity to FB and SRB. The SRB was more strongly inhibited by CC14 than FB at the same concentrations. CC14 of 0.001%~0.005% (V/V) showed little inhibition to FB,but the inhibition to SRB was above 47%~63%. Above 0.01% (V/V) of CC14 had very significant inhibition (96%~99%) to FB,but had much higher inhibition (99.9%) to SRB. FB was more tolerant to CC14 than SRB under the same concentrations. The different degree of microbial tolerance to CC14 will impact the effect of degradation and the products of transformation.

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