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Position: Home > Articles > Study on the Plant Tissue Culture Technology of Gynura procumbens(Lour.)Merr Anhui Agricultural Science Bulletin 2017 (14) 38-39+75

平卧菊三七组织培养技术研究

作  者:
沈春修;郑子峰
单  位:
宜春学院生命科学与资源环境学院
关键词:
平卧菊三七;组织培养;外植体
摘  要:
该研究以平卧菊三七的叶片为外植体,对影响其愈伤组织诱导及芽分化的相关因素进行了探讨。结果表明,适合平卧菊三七愈伤组织诱导的最佳培养基为MS+2,4-D 1mg/L;愈伤组织分化出芽的培养基配方为MS+6-BA 1mg/L+NAA 0.4mg/L。在1/2 MS+NAA 0.2 mg/L+IBA 0.3mg/L的培养基配方上生根较好,移栽到不同基质中的组培苗,存活率达100%。
译  名:
Study on the Plant Tissue Culture Technology of Gynura procumbens(Lour.)Merr
作  者:
Shen Chunxiu;College of Life Science Environment and Resource,Yichun University;
关键词:
Gynura procumbens(Lour.)Merr;;Tissue culture;;Explant
摘  要:
Taking leaf blade of Gynura procumbens(Lour.)Merr as explants material,the factors that influence the cal-lus induction and bud differentiation of Gynura procumbens(Lour.)Merr were studied.The results showed that the ini-tial medium for the callus induction was MS+2,4-D 1 mg/L;the best medium for adventitious was MS+6-BA 1 mg/L+NAA 0.4mg/L.The best subculture media for shoot proliferation was 1/2 MS+NAA 0.2 mg/L+IBA 0.3mg/L.Tissue cul-ture seedlings were transplanted to a different matrix,the survival rate was 100%.

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