摘 要：
建立红松种鳞中多酚HPLC指纹图谱分析方法。色谱条件为:C18反向色谱柱(200mm×4.6mm,5μm),以0.05%三氟乙酸溶液(A)和甲醇(B)作为流动相,洗脱梯度:0~11min,5%→15%B;11~16min,15%→17%B;16~23min,17%→23%B;23~36min,23%→35%B;36~50min,35%→40%B;50~65min,40%→50%B;65~70min,50%→5%B;流速为0.8mL/min,检测波长:280nm,该方法满足指纹图谱方法学考察。通过对8批样品的分析和比对,建立了红松多酚的指纹图谱,标示出14个稳定的共有峰,确定了咖啡酸为指纹图谱的参照峰。结果表明此方法简单、准确、重现性好,为红松多酚的质量控制标准提供了有效的方法。
译 名：
Fingerprints study on Pinus koraiensis polyphenols by HPLC
作 者：
FU Qun;GAO Qi;YU Wen-bin;XU Ming-hui;WANG Zhen-yu;College of Forestry,Northeast Forestry University;College of Food Engineering,Harbin Institute of Technology;
关键词：
Pinus koraiensis polyphenols;;HPLC;;gradient elution;;fingerprint
摘 要：
To establish the HPLC fingerprint of Pinus koraiensis polyphenols. The optimal chromatographic separating condition was:reverse C 18 column( 200mm × 4.6mm,5μm),the mobile phase was 0.05% trifluoroaceticacid( A)-methanol( B),elution gradient:0~11min,5% →15% B; 11~16min,15% →17% B; 16~23min, 17% →23% B; 23~36min,23% →35% B; 36~50min,35% →40% B; 50~65min,40% →50% B; 65~70min,50% →5% B; flow rate:0.8mL /min,the detection wavelength:( 280nm),this method satisfied the methodological study of the HPLC fingerprint.Based on the analysis and comparison of 8 batches of samples,eatablishing the HPLC fingerprint of Pinus koraiensis polyphenols. Marked 14 stable common peaks and determined coffee acid as the reference peak.It was concluded that this method was simple,accurate,reproducible and provided an effective method for the quality control standard of Pinus koraiensis polyphenol.
