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Position: Home > Articles > Study of mIκBα Expression Vector in Rats Hepatic Stellate Cells Journal of Yangtze University(Natural Science Edition)Agricultural Science Volumn 2009,6 (2) 5-6+18-21+28

mIκBα表达载体在大鼠肝星状细胞中的稳定表达

作  者:
杨友谊;邹伟敏
单  位:
湖北中医药高等专科学校;荆州区东城社区卫生服务中心
关键词:
肝星状细胞;核转录因子突变体;稳定转染
摘  要:
目的:探讨将mIκBα基因成功转染入大鼠肝星状细胞,并在其中稳定表达。方法:应用脂质体介导的基因转移技术将mIκBα基因真核表达载体pCMX-mIκBα导入大鼠肝星状细胞中,采用G418筛选途径得到稳定转染的大鼠肝星状细胞,再通过Western blot技术检测转染后大鼠肝形状细胞中相应mIκBα蛋白表达水平。结果:稳定转染的大鼠肝星状细胞中证实有pCMX-mIκBα相应蛋白质的表达。结论:mIκBα基因真核表达载体pCMX-mIκBα成功转入大鼠肝星状细胞,并在细胞中得到稳定表达,为进一步研究NFκB和肝星状细胞凋亡之间的关系,并为通过诱导肝星状细胞凋亡治疗肝纤维化寻找实验及理论基础。
译  名:
Study of mIκBα Expression Vector in Rats Hepatic Stellate Cells
作  者:
YANG You-yi (Hubei College of Chinese Medicine,Jingzhou 434020,China)ZOU Wei-min (Dongcheng Healthcare center of Jingzhou District,Jingzhou 434020,China)
关键词:
Hepatic stellate cells;mIκBα;Stable transfection
摘  要:
Objective:To confirm mIκBα expression in stable-transfected rats hepatic stellate cells.Methods: mIκBα expression vector pCMX-mIκBα was transfected into rats hepatic stellates.And the transfected cells were screened by G418,then the transfected and nontransfected cells were examined for mIκBα protein by Western blot analysis.Results: Expression of protein of pCMX-mIκBα were confirmed in the stable-transfected cells.Conclusion: mIκBα expression vector was successfully transfected into rats hepatic stellate cells and was stably expressed in rats hepatic stellate cells,which provides an experimental basis for exploring the role of NFκB in apoptosis of hepatic stellate cell and therapy of liver fibrosis.

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