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当前位置: 首页 > 文章 > 发酵藕带中优良乳酸菌的筛选及生理性能研究 长江蔬菜 2012 (8) 79-82
Position: Home > Articles > Research on Screening and Physiological Characteristics of Preferable Lactic Acid Bacteria from Fermented Lotus Sprout Journal of Changjiang Vegetables 2012 (8) 79-82

发酵藕带中优良乳酸菌的筛选及生理性能研究

作  者：
舒丽平;王清章;严守雷;李洁
单  位：
华中农业大学食品科技学院
关键词：
藕带;乳酸菌;亚硝酸盐;筛选鉴定
摘  要：
以水生蔬菜藕带为原料,从自然泡制藕带水中分离筛选出两株发酵藕带产酸能力强、亚硝酸盐含量低、风味好的乳酸菌菌株,生理生化鉴定为植物乳杆菌(Lactobacillus plantarum)和短乳杆菌(Lactobacillus brevis)。两菌株最适生长pH值为5.0~6.0,短乳杆菌在pH值3.0时生长良好,pH值大于8.0时生长受到抑制,植物乳杆菌在pH值3.0时生长很慢,而pH值大于9.0时仍能一定程度存活;在盐浓度低于6%时两菌株均能正常生长,10%含盐量抑制二者生长,植物乳杆菌能耐8%的盐浓度;两菌株生长温度范围较宽(20~37℃),最适生长温度均为30℃,在15℃时也能缓慢生长。在MRS液体培养基中,两菌株对初始浓度为100μg/mL的亚硝酸盐的降解率分别为89.65%和97.40%。
译  名：
Research on Screening and Physiological Characteristics of Preferable Lactic Acid Bacteria from Fermented Lotus Sprout
作  者：
SHU Liping,WANG Qingzhang,Yan Shoulei,LI Jie(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070)
关键词：
Lotus sprout;Lactic acid bacteria;Nitrite;Screening and identification
摘  要：
In this experiment,using the aquatic vegetable lotus sprout as raw material,two strains were isolated and screened from the natural fermentated lotus sprout pickle juice,which possessed the excellent characteristics of strong capability of acid production,low nitrite content and pleasant fermentation flavor,and they were identified as Lactobacillus plantarum and L.brevis by physiological and biochemical tests.The optimal pH value of the two strains was 5.0 to 6.0. L.brevis could grow well when the pH value was 3.0,but its growth was inhibited when the pH value was higher than 8.0.L.plantarum grew slowly when the pH value was 3.0,but it was still surviving to some extent when the pH value was higher than 9.0.Both the strains could grow normally when the salt concentration was below 6%,but their growth was restrained when the salt concentration was 10%.L.plantarum was tolerant of salt concentration of 8%.The two strains had considerably wide temperature tolerance,with the temperature ranged from 20℃ to 37℃,and the optimal temperature was 30℃,but they could grow slowly when the temperature was 15℃.L.plantarum and L.brevis depleted 89.65% and 97.40% nitrite in Man Rogosa Sharpe broth cultural medium,and the initial nitrite content of the cultural medium was 100 μg/mL.

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