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Position: Home > Articles > Determination of Organic Acids in Grape Wine Using Capillary Zone Electrophoresis FOOD SCIENCE 2009,30 (8) 209-211

毛细管电泳法测定葡萄酒中的有机酸含量

作  者:
唐美华;屠春燕;薛亚芳;严励
单  位:
南京工业大学学生工作处;南京工业大学制药与生命科学学院
关键词:
毛细管电泳;葡萄酒;有机酸
摘  要:
运用毛细管区带电泳法直接测定了葡萄酒中酒石酸、苹果酸、柠檬酸、琥珀酸、乳酸、乙酸、富马酸、草酸8种有机酸,并研究了缓冲液种类、缓冲液浓度、pH值、表面活性剂、电泳电压、电泳温度对分离度的影响。方法线性范围较宽,各有机酸线性相关系数在0.9990~0.9998之间,有机酸迁移时间及峰面积的RSD分别为0.68%~1.02%及3.68%~5.41%,方法回收率为91.9%~116.9%。方法简便、快速、准确,可对葡萄酒酿造过程快速监控,并对酒的品质进行监测。
译  名:
Determination of Organic Acids in Grape Wine Using Capillary Zone Electrophoresis
作  者:
TANG Mei-hua1,TU Chun-yan1,XUE Ya-fang1,YAN Li2(1.College of Life Science and Pharmacy Engineering,Nanjing University of Technology,Nanjing 210009,China;2.Department of Student Working,Nanjing University of Technology,Nanjing 210009,China)
关键词:
capillary zone electrophoresis;grape wine;organic acid
摘  要:
Eight organic acids(tartaric,malic,citric,succinic,lactic,acetic,fumarate and oxalic acids) in grape wine were directly determined by using capillary zone electrophoresis,and some important parameters including the types and concentrations of the running buffer,pH,surfactant,working voltage and working temperature were also systematically investigated.The linear range is wide,all of the correlation coefficients(r) are between 0.9990 and 0.9998,the relative standard deviation of migration time and peak area are 0.68% to 1.02% and 3.68% to 5.41% respectively,and the recovery ranges from 91.9% to 116.9%.The method is simple,rapid and accurate,and can quickly control winemaking process and detect wine quality.

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