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固定化纳米酶的研究进展

作  者:
肖鹭;周锦涛;刘振;熊涛;娄文勇;彭飞
单  位:
华南理工大学轻工与食品学院;南昌大学生命科学与食品工程学院;南昌大学食品学院
关键词:
固定化酶;纳米花;金属有机框架;纳米胶;纳米技术
摘  要:
游离酶因存在成本高、操作稳定性差、再利用困难等缺点在工业应用中受到限制,将其固定在载体上是克服上述缺点的有效手段。纳米酶固定化技术于近10年兴起,是一项具有巨大应用潜力的新兴技术。目前固定化酶已广泛应用于食品、医疗、环境治理等领域。本文系统且深入地综述基于蛋白-无机杂化纳米花、金属有机框架和纳米胶3种新型纳米材料固定化酶的最新研究与应用进展,综合比较三者的优缺点,并分析总结相应的适用酶,最后提出级联酶固定化和新型固定化材料开发是固定化酶未来的发展趋势。
作  者:
XIAO Lu;ZHOU Jintao;LIU Zhen;XIONG Tao;LOU Wenyong;PENG Fei;School of Food Science and Engineering, Nanchang University;College of Food Science and Engineering, South China University of Technology;
关键词:
immobilized enzyme;;nanoflowers;;metal-organic frameworks;;nanogels;;nanotechnology
摘  要:
The industrial application of free enzyme is limited due to its high cost, poor operational stability and difficult reuse. Immobilization on carrier is an effective way to overcome the limitation. Nanozyme immobilization technology has emerged in the past 10 years, which is an emerging technology with great potential. At present, immobilized enzymes have been widely used in several fields such as food, medical treatment, and environmental treatment. This article systematically summarizes the latest progress in the research and application of immobilized enzymes based on three new nanomaterials(protein-inorganic hybrid nanoflowers, metal organic frameworks and nanogels), comprehensively compares the advantages and disadvantages of the three nanomaterials, analyzes and summarizes their respective applicable enzymes, and finally points out that cascade enzyme immobilization and the development of new immobilized materials are the future trend for the development of immobilized enzymes.

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