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Position: Home > Articles > Study on Immobilization ofα-Amylase with 3-Aminopropyl Silica Gel as Carrier FOOD SCIENCE 2009,30 (1) 169-172

氨基化硅胶载体固定化α-淀粉酶的研究

作  者：
刘海燕;李宁;李敬慈;吴秋华;段慧云;杨秀敏
单  位：
河北大学化学与环境科学学院;河北农业大学理学院
关键词：
α-淀粉酶;固定化酶;3-氨丙基硅胶
摘  要：
采用5种硅胶作为载体,戊二醛作偶联剂,制备了固定化α-淀粉酶。对制备固定化α-淀粉酶的硅胶目数、偶联剂浓度、温度、pH值的影响因素进行了研究和优化。并对固定化酶和游离酶的酶学性质进行了比较。结果表明:固定化酶的最适温度为75℃,比游离酶提高了10℃,固定化酶的热稳定性优于游离酶。与游离酶相比,其对酸碱的适应性、贮存稳定性、操作稳定性也都有较明显改善。固定化酶的K_m值略低于游离酶,前者为2.13mg/ml,后者为1.02mg/ml。
译  名：
Study on Immobilization ofα-Amylase with 3-Aminopropyl Silica Gel as Carrier
作  者：
LIU Hai-yan~(1,2) LI Ning~(1,3) LI Jing-ci~1 WU Qiu-hua~1 DUAN Hui-yun~2 YANG Xiu-min~1 1.College of Science,Agricultural University of Hebei,Baoding 071001,China; 2.College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China; 3.College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China
关键词：
α-amylase;;immobilized enzyme;;3-aminopropyl silica gel
摘  要：
α-Amylase was immobilized on five 3-aminopropyl silica gels with glutaraldehyde as crosslinking agent.Some factors that affect the activity of immobilizedα-amylase were investigated,such as the mesh of silica gel,concentration of glutaraldehyde,reaction temperature and pH.The propertiees of imm obilized and freeα-amylases were also compared.The results indicated that the optimum temperature of the immobilized enzyme is 75℃,which is 10℃higher than that of the freeα-amylase, and its thermal stability is better than that of free enzyme.Compared with fleeα-amylase,the immobilizedα-amylase is also markedly improved in acid and alkaline stability,storage stability and operation stability.Furthermore,the Michealis constant (K_m)of immobilizedα-amylase is 2.13 mg/ml,while that of freeα-amylase is 1.02 mg/ml.

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