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Position: Home > Articles > Experiment Study of Nisin Producing on Whey by Streptococcus Lactis P-99 Transactions of the Chinese Society for Agricultural Machinery 2007,38 (11) 75-79

乳链球菌P-99发酵乳清生产乳链菌肽的研究

作  者:
罗水忠;潘利华;潘丽军
关键词:
乳清;乳链菌肽;发酵动力学;细菌素
摘  要:
研究了乳清培养基中乳链球菌P-99的细胞生长和乳链菌肽合成特性,采用正交试验对乳清发酵培养基中的碳源、氮源和磷源进行了优化。结果表明,乳链球菌P-99能够在没有添加物的乳清培养基中生长并合成乳链菌肽,其细胞生长和产物合成可以分别用Monod方程和Luedeking-Piret方程拟合。培养基优化试验结果表明,在乳清发酵培养基中添加19g/L乳糖、0.8g/L甘氨酸、0.45g/LKH2PO4时,乳链菌肽的合成量提高了31.7%。
译  名:
Experiment Study of Nisin Producing on Whey by Streptococcus Lactis P-99
作  者:
Luo Shuizhong Pan Lihua Pan Lijun(Hefei University of Technology)
关键词:
Whey, Nisin, Fermentation kinetics, Bacteriocin
摘  要:
In this paper, the characteristics of cell growth and nisin production on whey by Streptococcus lactis P-99 were investigated, and concentrations of carbon source, nitrogen source and phosphorus source in whey medium were optimized. The results showed that Streptococcus lactis P-99 could grow well and produce nisin on whey medium without any adjuncts. Based on Monod equation and Luedeking-Piret equation, the kinetic model describing cell growth and nisin production was established. The producing rate of Nisin was improved by 31.7% when 19 g/L lactose, 0.8 g/L glycine, 0.45 g/L KH2PO4 were added to the whey medium.

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