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当前位置: 首页 > 文章 > 鸡Slit2和Robol基因mRNA组织表达特性分析 中国家禽 2013,35 (14) 6-10
Position: Home > Articles > Analysis of mRNA Expression Profiles of Slit2 and  Robol Genes in Some Tissues of Chickens China Poultry 2013,35 (14) 6-10

鸡Slit2和Robol基因mRNA组织表达特性分析

作  者：
姜建萍;徐日福;范贤聪;张英英;刘强;杨雨江
单  位：
吉林农业大学动物科学技术学院
关键词：
mRNA表达水平;海兰白蛋鸡;肝脏组织;Robo;l基因;表达特性;PCR方法;半定量RT
摘  要：
SLIT/ROBO信号通路在鸡的卵泡发育过程中发挥着重要作用,Slit2和Robol基因是影响鸡产蛋性能的关键候选基因.本试验以大骨鸡和海兰白蛋鸡为供试素材,利用半定量RT-PCR方法对Slit2和Robol基因在鸡不同组织中mRNA表达水平进行测定分析.结果显示,Slit2和Robol基因mRNA在卵巢和输卵管等8种组织中均呈较广泛的分布(除在海兰白胸肌、腿肌中未检测到扩增条带外);Robol基因mRNA相对表达量除在脾脏和肺脏组织中的表达水平相对较低(P〈0.01)外,在肝脏、卵巢和输卵管3种组织中的mRNA表达量均维持于较高水平(P〈0.01).虽然在被检各组织中的Slit2基因mRNA相对表达量存在一定的差异(P〈0.05),但在卵巢等其它7种组织中的表达水平均较高(除在海兰白肝脏组织中的表达较低外).在被检8种组织中Robol和Slit2基因mRNA相对表达量在每一品种各分别呈现出基本一致的变化趋势.
译  名：
Analysis of mRNA Expression Profiles of Slit2 and  Robol Genes in Some Tissues of Chickens
关键词：
Dagu chicken% Hy-line White layer%Sl#2 gene%Robol gene% mRNA expression
摘  要：
The SLIT/ROBO pathway plays a pivotal role during the development of ovary follicles in chicken,and Slit2 and Robol genes are the key candidate genes affectting egg laying performance. In the current study,various tissues from Dagu chicken and Hy-line White layers were used to investigate the mRNA expression profiles of Slit2 and Robol genes by semi-quantitative RT-PCR. The results showed that the high levels of mRNA expression of Slit2 and Robol genes were observed in all tissues detected except in the muscle tissues of Hy-line White layers. Between the Dagu chicken and Hy-line White layers, the levels of Robol gene mRNA expression were observed significantlyhigher in three tissues including liver, ovary and oviduct (P〈0.01), while the lower levels of the mRNA were detected in spleen and lung tissues (P〈0.01). Although the significant differences in the levels of Slit2 gene mRNA expression were also observed in some tissues out of the eight tissues detected in Dagu chicken and Hy-line White (P〈0.05), the significant higher levels of mRNA expression of Slit2 and Robol genes in the seven tissues including spleen, lung,thigh,ovary, oviduct,kidney and pectoralis muscles,were observed in the chickens (P〈0.05),respectively. The changes of the levels of mRNA expression of Slit2 and Robo! genes appeard to be consistent in a concerted manner in the same species and tissues.

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