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Position: Home > Articles > Establishment of PCR-Based Technique for Tracing Spoilage Microorganisms in Meat Products FOOD SCIENCE 2013,34 (14) 178-181

肉制品致腐微生物溯源技术构建

作  者:
陈晓;高晓平;李苗云;柳艳霞;赵改名;孙灵霞;张秋会;张建威;黄现青
单  位:
河南农业大学食品科学技术学院,河南省肉制品加工与质量安全控制重点实验室;河南农业大学食品科学技术学院
关键词:
溯源;特异性;灵敏性;地衣芽孢杆菌
摘  要:
针对热杀菌肉制品中因微生物残留而引起的腐败问题,选择从腐败的玉米热狗肠中分离出并由Biolog微生物自动分析系统鉴定的地衣芽孢杆菌为代表菌株,结合地衣芽孢杆菌的特异性引物,用聚合酶链式反应(PCR)方法构建致腐微生物溯源技术。结果表明,该方法可在腐败样品中准确、灵敏、快速地检出地衣芽孢杆菌,引物的特异性为100%,方法的检测限为0.104CFU/g。
译  名:
Establishment of PCR-Based Technique for Tracing Spoilage Microorganisms in Meat Products
作  者:
CHEN Xiao,GAO Xiao-ping,LI Miao-Yun,LIU Yan-xia,ZHAO Gai-ming,SUN Ling-xia,ZHANG Qiu-hui,ZHANG Jian-wei,HUANG Xian-qing*(Henan Key Laboratory of Meat Processing and Quality Safety Control,College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002,China)
关键词:
specificity;sensitivity;Bacillus licheniormis
摘  要:
Thermally sterilized meat products may still become spoiled due to the presence of residual microorganisms.In the present study,a PCR-based technique was developed for tracing spoilage microorganisms in meat products.For this,a set of specific primers was designed for Bacillus licheniormis as a representative strain of meat spoilage microorganisms,isolated from spoiled corn hot dog sausage and identified with the Biolog microbial identification system.The PCR-based method allowed accurate,sensitive and rapid detection of Bacillus licheniormis in spoiled samples with a 100% primer specificity and a detection limit of 0.104 CFU/g.

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