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Position: Home > Articles > Acute toxicity test of arecoline hydrobromide tablets for oral usein mice and rats Heilongjiang Animal Science and Veterinary Medicine 2012 (21) 114-115

大鼠及小鼠口服氢溴酸槟榔碱片剂的急性毒性试验

作  者:
周绪正;张继瑜;李金善;李剑勇;魏小娟;牛建荣;李冰
单  位:
中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室
关键词:
氢溴酸槟榔碱;小鼠;大鼠;口服;急性毒性
摘  要:
为了研究氢溴酸槟榔碱片剂对小鼠及大鼠的口服急性毒性,试验以小鼠及大鼠为实验动物,经口一次性灌胃给药,统计死亡率,用改良寇氏法计算半数致死量(LD50)并观察毒性作用。结果表明:氢溴酸槟榔碱片剂对小鼠的口服LD50为6 545 mg/kg,其95%可信区间为5 534~7 740 mg/kg;对大鼠的最大耐受量(LD0)大于10.0 g/kg,则其LD50必大于10.0 g/kg。说明氢溴酸槟榔碱片剂属于低毒性的或实际无毒的药物,以20~50 mg/kg的剂量应用于临床非常安全。
译  名:
Acute toxicity test of arecoline hydrobromide tablets for oral usein mice and rats
作  者:
ZHOU Xu-zheng,ZHANG Ji-yu,LI Jin-shan,LI Jian-yong,WEI Xiao-juan,NIU Jian-rong,LI Bing(Key Laboratory of New Animal Drug Project,Gansu Province/ Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture/ Lanzhou Institute of Animal Science and Veterinary Pharmaceutics Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China)
关键词:
arecoline hydrobromide;mice;rats;oral use;acute toxicity
摘  要:
To study the acute toxicity of arecoline hydrobromide tablets for oral use in mice and rats,the mice and rats was selected and fed the medicine with one-time oral gavage,and then the mortality rate of the mice was counted.The toxic effect was observed and the LD50 were calculated by Karber method.The results showed that the LD50 of arecoline hydrobromide tablets in mice was 6 545 mg/kg,and 95% confidence interval of its LD50 was 5 534 to 7 740 mg/kg.The LD0 of arecoline hydrobromide tablets in rats exceed 10.0 g/kg;and the LD50 of arecoline hydrobromide tablets in rats must be greater than 10.0 g/kg.The results indicate that arecoline hydrobromide tablets belong to low-toxicity or actual non-toxic medicine,the dose of 20 to 50 mg/kg is very safe in the clinical application.

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